Inflammatory Bowel Disease (IBD)
Conditions
Keywords
Monocytes, Metabolic reprogramming
Brief summary
Inflammatory bowel diseases, Crohn's disease and haemorrhagic rectocolitis, are pathologies that progress in flare-ups, impacting on patients' quality of life and functional or even vital prognosis. These inflammatory diseases require the use of immunosuppressive and immunomodulatory treatments, the side-effects of which can be significant, and the limited number of which sometimes puts patients and practitioners in a therapeutic impasse from which surgery is the only way out. It is therefore important to be able to develop new therapeutic approaches, ideally better tolerated, that can control inflammation during relapses. Monocytes are one of the main players in the inflammatory reaction. In the laboratory, we have developed a strategy for the metabolic reprogramming of these cells based on the use of oxygen microbubbles to modulate the inflammatory response of monocytes.
Interventions
BIOLOGICALBlood test 4 EDTA tubes
Blood test 4 EDTA tubes for biological check-up
Sponsors
University Hospital, Grenoble
Institute for Advanced Biosciences (IAB), Grenoble
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Men and women over 18 * Managed at the CHUGA for a severe IBD flare-up requiring hospitalisation or endoscopy for flare-up * Patient not objecting to the REPRO-MICI study
Exclusion criteria
* Patients protected by law (pregnant or breast-feeding women, minors, patients under guardianship or trusteeship, persons deprived of their liberty or hospitalised under duress). * Patients with positive HIV, HBV or HCV serology. * Patients with a positive ELISPOT with no history of treatment for latent tuberculosis.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Comparaison of reduction of concentration of inflammatory cytokines and chemokines in cells treated by oxygen microbubbles vs cells not treated | up to 2 years | Validation of inhibition of the production of inflammatory cytokines and chemokines by the monocytes treated by oxygen microbubbles |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Comparaison of tissue factor expression at the membrane of monocytes treated by microbubbles vs monocytes not treated | up to 2 years | Inhibition of tissue factor expression at the membrane of treated monocytes |
| Comparaison of glucose metabolism rate in monocytes treated bu microbubbles vs not treated cells | up to 2 years | Modulation of glucose metabolism in treated monocytes |
Countries
France
Contacts
CONTACTMarianne HUPE, MD PhD
CONTACTAnna Borowik, PhD
PRINCIPAL_INVESTIGATORMarianne HUPE, MD PhD
Grenoble Alpes University Hospital
Outcome results
None listed