Anaerobic Performance, Sports Nutrition, Exercise Recovery, Muscle Fatigue, Creatine Supplementation, Recreational Physical Activity, Female Athletes
Conditions
Keywords
Creatine Monohydrate, Pre-Sleep Supplementation, Anaerobic Power, Wingate Test, Muscle Damage Markers, Creatine Kinase (CK), Lactate Dehydrogenase (LDH), Female Exercise Performance, Nutritional Ergogenic Aid, Resistance Training, Recovery Enhancement
Brief summary
This study is a randomized, double-blind, placebo-controlled trial designed to examine the effects of pre-sleep creatine monohydrate supplementation on anaerobic performance and muscle damage markers in recreationally active females. Participants will be randomly assigned to receive either 5 grams of creatine monohydrate or a placebo 30 minutes before sleep for seven consecutive days. Anaerobic performance will be assessed using the Wingate Anaerobic Test, evaluating peak and average power output and fatigue index. Blood samples will be collected before and after the supplementation period to analyze markers of muscle damage, including creatine kinase and lactate dehydrogenase. This study aims to provide insight into the efficacy of short-term, time-specific creatine supplementation for enhancing performance and recovery.
Interventions
DIETARY_SUPPLEMENTCreatine Monohydrate
Participants will receive 5 grams of creatine monohydrate powder dissolved in water, flavored with a sugar-free agent, 30 minutes before sleep each night for 7 days.
DIETARY_SUPPLEMENTPlacebo (Maltodextrin)
Visually identical placebo (maltodextrin powder, 5 g/day), flavored with a sugar-free agent, dissolved in water and consumed 30 minutes before sleep for 7 consecutive days.
Sponsors
Ankara Yildirim Beyazıt University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Masking description
Neither the participants nor the investigators know which intervention the participant is receiving.
Intervention model description
Participants are randomly assigned to one of two groups: a creatine supplementation group or a placebo group, and they remain in that group for the duration of the trial.
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 25 Years
Healthy volunteers
Yes
Inclusion criteria
Female, aged 18 to 25 years Recreationally active (engaging in structured resistance training 3-4 times per week for the past 6 months) Regular menstrual cycles (used for scheduling purposes) Able and willing to provide informed consent Agreement to maintain normal diet and training routines throughout the study
Exclusion criteria
History of musculoskeletal injury affecting lower or upper limbs within the past 6 months Known allergy or intolerance to creatine or maltodextrin Use of performance-enhancing substances or supplements within the last 30 days Use of recovery modalities such as massage, cryotherapy, or compression garments during the study period Current use of anti-inflammatory medication Pregnancy or breastfeeding Inability to comply with study protocol or scheduled assessments
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Average Power Output (Watts) - 30-Second Wingate Test | Baseline and post-intervention (Day 0 and Day 7) | Average anaerobic power output will be calculated across the 30-second Wingate cycling test. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Peak Power Output (Watts) - Wingate Test | Baseline and post-intervention (Day 0 and Day 7) | Peak power output during the initial 5 seconds of the 30-second Wingate cycling test. |
| Fatigue Index (%) - Wingate Test | Baseline and post-intervention (Day 0 and Day 7) | Calculated as the percent drop in power from peak to minimum power output during the 30-second Wingate test, indicating fatigue rate. |
| Serum Creatine Kinase (CK) Activity | Baseline and 24 hours post Wingate Test (Day 0 and Day 8) | Serum creatine kinase activity will be measured as a marker of muscle damage using a spectrophotometric assay. |
| Serum Lactate Dehydrogenase (LDH) Activity | Baseline and 24 hours post Wingate Test (Day 0 and Day 8) | Serum lactate dehydrogenase activity will be measured as an indicator of cell membrane permeability and muscle damage using spectrophotometry. |
Countries
Iran
Contacts
Primary ContactMorteza Jourkesh, PhD
Backup ContactCaglar Soylu, phD
Outcome results
None listed