Skin Health
Conditions
Keywords
Ozone reaction products, Skin health
Brief summary
This is a randomized controlled human exposure study. Investigators aim to investigate the products of ozone-sebum reactions and their acute effects on skin health of healthy young adults.
Detailed description
The investigators will conduct a randomized, controlled human exposure study involving 40 healthy young adults in Shanghai, China. Each participant will have both forearms simultaneously exposed-one in a clean air chamber and the other in an ozone chamber-for 2 hours. The ozone concentration at the ozone chamber inlet will be maintained at 200 ppb. The temperature and relative humidity in both chambers will be maintained at 27±2℃ and 45%±5%, respectively. Health examinations will be conducted at three time points: immediately before exposure, immediately after exposure, and 24 hours after exposure. These examinations will include skin measurements and sampling.
Interventions
OTHEROzone exposure
Participants' forearms were exposed to 200 ppb ozone for 2 h at a temperature and relative humidity of 27±2°C and 45%±5%, respectively.
OTHERClean air exposure
Participants' forearms were exposed to clean air for 2 h at a temperature and relative humidity of 27±2°C and 45%±5%, respectively.
Sponsors
Fudan University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* Living in Shanghai during the study period; * Having healthy skin conditions on both forearms.
Exclusion criteria
* Subjects with a history of smoking and alcohol abuse; * Subjects with a regular use of medication and dietary supplements within 2 months prior to enrollment; * Subjects with severe skin diseases, such as atopic dermatitis, eczema, or severe acne; * Subjects with allergic diseases, such as allergic rhinitis, allergic asthma, or atopy, or history of allergic reactions within 2 months prior to enrollment; * Subjects with cardiovascular diseases, such as congenital heart disease, pulmonary heart disease, or hypertension; * Subjects with respiratory diseases, such as asthma, acute or chronic bronchitis, or pneumonia; * Subjects with chronic diseases, such as diabetes, chronic hepatitis, or kidney disease; * Subjects with cutaneous abnormalities on forearm, such as severe pigmentation, multiple nevus, or tattooing; * Subjects who underwent cosmetic intervention on forearms within 2 months prior to enrollment, such as whitening treatments, UV exposure therapies, depigmentation procedures, or tanning; * Females in pregnancy or lactation status.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes in chemical exposome level on the forearm skin | Immediately before exposure and immediately after exposure. | Changes in the amount and level of chemical contaminants between ozone and clean air exposures, which will be detected using non-targeted or targeted methods. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in skin hydration | Immediately before exposure, immediately after exposure, and 24 hours after exposure. | Changes in the amount of skin hydration, which will be measured by a hydration meter. |
| Changes in TEWL | Immediately before exposure, immediately after exposure, and 24 hours after exposure. | Changes in transepidermal water loss on the forearm, which will be measured by an evaporimeter. |
| Changes in pH | Immediately before exposure, immediately after exposure, and 24 hours after exposure. | Changes in the pH level of skin, which will be measured by a PH meter. |
Countries
China
Contacts
STUDY_DIRECTORYue Niu, PhD
Department of Environmental Health, School of Public Health, Fudan University
Outcome results
None listed