Glucose, Immune System and Related Disorders, Isotope Labeling, Metabolism
Conditions
Brief summary
The purpose of this study is to understand how cells of the immune system use the common sugar glucose to fuel energy production and as a building block within the cell. Investigators will intravenously infuse a non-radioactive glucose tracer into participants over a few hours and collect immune cells from the blood to track uptake and usage of this glucose within these immune cells.
Interventions
DRUG13C6-Glucose
Compounded as 5% 13C6-Glucose in sterile water given IV over 2-4 hours as a split bolus and infusion
Sponsors
Vanderbilt University Medical Center
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Adults aged 18 or older
Exclusion criteria
* Pregnant * Prisoner or in police custody * Uncontrolled hyperglycemia/diabetes * Current participation in another study where the total volume of blood drawn, when added to this study blood draw volume, exceeds 500cc in an 8 week period * Any medical issue or pharmacologic exposure which, in the opinion of the study investigator, that might interfere with the study objectives * Any reason which, in the opinion of the study investigator, adds additional risk to the participant
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 13C6-glucose uptake in immune cells | Up to 4 hours | Detection of 13C6-glucose-derived metabolites in neutrophils, monocytes, CD4 T cells, and CD8 T cells using gas and liquid chromatography-mass spectrometry (GC-MS and LC-MS) following magnetic enrichment of these immune cell subsets. |
Countries
United States
Contacts
Primary ContactMatthew T Stier, M.D., Ph.D.
Outcome results
None listed