Oxygen Saturation
Conditions
Brief summary
The purpose of this study is to compare oxygen saturation measurement readings from manufactured pulse oximetry devices to the oxygen saturation measurements of arterial blood samples drawn at the same time of measurements.
Interventions
PROCEDUREFinger hyperperfusion via adenosine
Subjects will be fitted with a mouthpiece and/or facemask to systematically reduce the fraction of inspired oxygen (FiO2) they breathe, starting with room air (21% O2), and then progressive hypoxic mixtures of 18%, 15%, 12%, and 10% FiO2 in sequence for five minutes each. Blood flow to the hand downstream from the brachial catheter will be increased by brachial artery infusions of adenosine administered at 6.25 mcg/100ml tissue per minute for 30 minutes into the brachial artery to increase blood flow by 5 to 10-fold.
PROCEDUREFinger hypoperfusion via by norepinephrine
Subjects will be fitted with a mouthpiece and/or facemask to systematically reduce the fraction of inspired oxygen (FiO2) they breathe, starting with room air (21% O2), and then progressive hypoxic mixtures of 18%, 15%, 12%, and 10% FiO2 in sequence for five minutes each. Blood flow to the hand downstream from the brachial catheter will be decreased to \~25% of baseline, by infusing norepinephrine at 4 ng/100ml of tissue per minute for 30 minutes.
PROCEDUREBaseline hypoxic test
Subjects will be fitted with a mouthpiece and/or facemask to systematically reduce the fraction of inspired oxygen (FiO2) they breathe, starting with room air (21% O2), and then progressive hypoxic mixtures of 18%, 15%, 12%, and 10% FiO2 in sequence for five minutes each.
Sponsors
Mayo Clinic
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy adult men and women of all races and ethnic backgrounds recruited from faculty and staff of the Mayo Medical Center and from Rochester, MN and surrounding communities. * Equal number of male and female participants to address potential sex differences in physiology. * We will intentionally recruit subjects with dark skin because of the issues surrounding accuracy of pulse oximetry and skin tone. * Non-smokers * BMIs between 18-30 kg·m-2. * Female subjects will be non-pregnant. * Free of cardiopulmonary diseases or other conditions and/or taking medications that might affect pulse oximeter-based estimates of arterial saturation. * All inclusion and
Exclusion criteria
are at the discretion of the Principal Investigator. * At least 8 of the subjects will have Fitzpatrick Skin Type of IV to VI.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Arterial oxygen saturation measured via arterial blood gas sample (Sa02) | 30 minutes, 90 minutes, 3 hours | Arterial oxygen saturation will be obtained via a blood sample at the end of each stage of the breath down protocols and is reported as a percentage. |
| Arterial oxygen saturation measured via pulse oximeters (Sp02) | 30 minutes, 90 minutes, 3 hours | Arterial oxygen saturation will be obtained via pulse oximeter reading at the end of each stage of the breath down protocols and is reported as a percentage. |
Countries
United States
Contacts
CONTACTNancy Meyer
PRINCIPAL_INVESTIGATORMichael Joyner, MD
Mayo Clinic
Outcome results
None listed