Anxiety Symptoms, Depressive Symptoms, Early Life Adversity, Microbiome, Human, Posttraumatic Stress Symptoms
Conditions
Keywords
early life adversity, gut microbiome, anxiety symptoms, depressive symptoms, posttraumatic stress symptoms
Brief summary
The goal of this observational study is to learn about the effects of early-life adversity (ELA) on the composition of children's microbiome and on their psychosocial functioning. The main questions it aims to answer are: * Do children who have experienced ELA have lower gut microbial diversity and/or an altered gut microbial composition? * Do these microbiome alterations correlate with adverse neurodevelopmental outcomes, including increased levels of stress, social and/or affective problems?
Interventions
DIAGNOSTIC_TESTCompletion of questionnaires; child psychiatric screening; faecal sample analysis
Completion of questionnaires: * FFQ (Food Frequency Questionnaire) - completed by child * KIDSCREEN-10 - completed by child * CRIES-13 (Child Revised Impact of Events Scale) - completed by parents/guardian and child * CTES (Childhood Trust Events Survey) - completed by parents/guardian * RCADS-25 (Revised Children's Anxiety and Depression Scale) - completed by parents/guardian and child Child psychiatric screening using SCID-5-Junior - semi-structured interview conducted with child and parent(s)/guardian Faecal sample analysis: * Beta-diversity (UniFrac and Bray-Curtis distance index) * Firmicutes/Bacteroidetes ratio * Abundance of Prevotella, Lactobacillaceae, Bifidobacteriacae and Lachnospiraceae * Short-chain fatty acid metabolites
Sponsors
Universitair Ziekenhuis Brussel
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
6 Years to 13 Years
Healthy volunteers
No
Inclusion criteria
* Primary school-aged children (6 to 13 years of age) who have experienced at least one early-life traumatic event, based on the Childhood Trust Events Survey (CTES) questionnaire * The child and at least one of his/her parents (or guardian) speak Dutch sufficiently well to complete all of the questionnaires and the semi-structured child psychiatric interview (SCID-5-Junior)
Exclusion criteria
* any chronic disorders or diseases known to affect the gut micobiome * inablity to complete questionnaires and/or semi-structured interview because of linguistic and/or cognitive barrier * congenital or acquired immunodeficiencies * use of medication(s) that affect gastrointestinal function (e.g., antibiotics) within the last 6 weeks, or laxatives within the last 4 weeks, or probiotic or prebiotic supplements within the last 4 weeks * current involvement in another clinical or food study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Global mental wellbeing | Assessment at enrollment | KIDSCREEN-10 total score |
| Microbiomial diversity | Assessment based on single stool sample, within 4 weeks after enrollment | Gut microbiomial beta-diversity, based on UniFrac and Bray-Curtis distance index |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Post-traumatic stress symptoms | Assessment at enrollment | CRIES-13 (Child Revised Impact of Events Scale) total scores, based on parent version and based on child version |
| Gut microbiomial composition | Assessment based on single stool sample, within 4 weeks after enrollment | Gut microbiomial abundance of Prevotella, Lactobacillaceae, Bifidobacteriacae and Lachnospiraceae |
| Firmicutes/Bacteroidetes ratio | Assessment based on single stool sample, within 4 weeks after enrollment | Gut microbiomial Firmicutes/Bacteroidetes ratio |
| Anxiety and depression symptoms | Assessment at enrollment | RCADS-25 (Revised Children's Anxiety and Depression Scale) total scores, based on parent version and child version |
| Short-chain fatty acid metabolites | Assessment based on single stool sample, within 4 weeks after enrollment | Gut microbiomial short-chain fatty acid metabolites |
Countries
Belgium
Contacts
Primary ContactSam L.B. Bonduelle, Master of Medicine
Outcome results
None listed