Hypopigmentation
Conditions
Brief summary
Hypopigmented skin changes are commonly encountered by dermatologists. A new dermatological entity was identified as scattered, hypopigmented oval-shaped macules and patches distributed mostly in seborrheic area of the face and of the trunk in dark skinned individuals. This patterned presentation of hypopigmentation was first described in the literature under the name of hypochromic vitiligo or vitiligo minor. Nerveless, histopathological patient's specimens analyzed by Krueger et al. clearly highlight that there is no tangible causal correlation with a diagnosis of vitiligo. They propose to rename this entity to Seborrheic Macular Hypopigmentation (SMH). The etiopathology of this dermatosis is still unknown preventing to propose any satisfactory treatment for this disfiguring affection.The objective of this study is to analyze the bacterial and fungal skin microbiome on hypochromic lesions of SMH compared to the surrounding non-lesional skin of the same patients and to healthy volunteers
Interventions
OTHERSkin's lesion patients
Skin swabs will be obtained from the patient's facial lesional (FLS) and perilesional sites (FPS) using a sterile cotton swab. The microbiota of the same anatomical face regions will be collected on volunteers, once the 10 SMH patients have been enrolled. The swab is premoistened in a specific cocktail solution and rubbed onto the skin surface for 45sec. The pressure during the sampling involves rubbing the area of interest with a smear over the entire zone. The skin reddens slightly under the exerted pressure.
OTHERSkin's lesion vulunteers
Skin swabs will be obtained from the patient's facial lesional (FLS) and perilesional sites (FPS) using a sterile cotton swab. The microbiota of the same anatomical face regions will be collected on volunteers, once the 10 SMH patients have been enrolled. The swab is premoistened in a specific cocktail solution and rubbed onto the skin surface for 45sec. The pressure during the sampling involves rubbing the area of interest with a smear over the entire zone. The skin reddens slightly under the exerted pressure.
Sponsors
Centre Hospitalier Universitaire de Nice
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Intervention model description
The study schedule consists of two on-site visit (inclusion and followup). Skin swabs will be obtained from the patient's facial lesional (FLS) and perilesional sites (FPS) located in the same facial area but at least 1 cm away from any hypopigmented macules. One skin swab will be obtained in healthy volunteers matched on sex, age, skin type and location of the skin sample.
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
1. Female and male patients ≥ 18 years of age 2. Clinical diagnosis of SMH with lesion of the face 3. Written informed consent obtained before any assessment is performed 4. Affiliation with a social security scheme 5. Physical and psychological ability to participate
Exclusion criteria
1. Use of systemic antibiotics or any systemic antifungal treatments within 4 weeks before the inclusion visit 2. Use of topical antibiotics or any topical antifungal treatments on the face within 2 weeks before the inclusion visit 3. Use of any topical cream, gel, serum or ointment within 2 days before the inclusion visit 4. Laser, radiofrequency, peeling or any other procedure on the face in the past 3 months before the inclusion visit 5. Vulnerable patients: minors, patients under guardianship or curatorship, pregnant or breast-feeding women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The bacterial skin microbiome of SMH patients vs voluntheers | at baseline | For each sample, the bacterial profiles determined by real time PCR, will be described and compared as follows: seborrheic macular hypopigmented lesions versus same anatomic area in healthy volunteers. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The bacterial skin microbiome of SMH patients compare to the non-lesional surrounded skin of the same patients. | at baseline | For each sample, the bacterial profiles determined by real time PCR, will be described and compared as follows: intra-individual hypochromic macula lesions versus perilesional areas located at least 1cm away from any hypopigmented macules. |
Countries
France
Contacts
Primary ContactThierry Passeron, PhD
Backup Contactemmanuelle PRADELLI
Outcome results
None listed