There Are no Conditions Under Study. Healthy Volunteers
Conditions
Keywords
Eccentric cycling exercise, Neuromuscular electrical stimulation, Compressive garment, Lymphocyte, Mitochondria
Brief summary
Eccentric cycling exercise can be a promising modality fwith lower respiratory demands and higher power output. Assistive devices like neuromuscular electrical stimulation (NMES) and compressive garment (CG) accelerates muscle pumping and relieve the eccentric exercise-induced muscle soreness. Phenotypes and mitochondrial metabolism are crucial to regulate acquired immnunity of T lymphocytes. The study aimed to investigate the effects of eccentric cycling exercise training combined with NMES and CG on mitochondrial phenotypes and bioenergetics in T lymphocytes in sedentary males. The hypothesis were an acute/intense exercise decreased the mitochondrial respiratory capacities of OXPHOS, ETS and bioenergetics, while eccentric cycling training could elevate the diminished mitochondrial functions. Methods: Twelve sedentary healthy males were randomly assigned to either eccentric cycling with assistive devices (ECA, n=10) or eccentric cycling only (ECC, n=10). All participants performed ECA or ECC at an intensity of 60% to 120% maximal workload progressively on a costumed bicycle ergometer for 40 min/day, 5 days/week for 5 weeks. ECA performed NMES for 20min/session after cycling, while ECC was seated for resting 20 min. Systemic aerobic fitness, phenotypes of T lymphocytes, mitochondrial OXPHOS, ETS respiratory capacities, and bioenergetics in T lymphocytes were analyzed.
Interventions
BEHAVIORALEccentric cycling exercise (ECC)
Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. After each training session, rest in sitting position for 20 min. Exercise intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.
BEHAVIORALEccentric cycling exercise with assistive devices (ECA)
Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. After each training session, receive NMES in sitting position for 20 min. Intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.
Sponsors
Chang Gung Memorial Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
20 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* Sedentary lifestyle (exercise\<20min/session; \<1session/week for a year or longer). * Nonsmokers, nonusers of medications/vitamins. * No any cardiopulmonary, neuromuscular, and hematological risks.
Exclusion criteria
* Have regular exercise habits (i.e., exercise frequency once per week, duration \>20 min). * Have retabolic or musculoskeletal disease.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mitochondrial bioenergetics of oxygen consumption rate in T lymphocyte | At baseline and 2 days after completing the 5-week intervention | The bioenergetic health index (BHI) of mitochindria in T lymphocyte was measured. BHI is a parameter for detecting the health status of mitochondrial and efficiency of energy metabolism. |
| Systemic aerobic fitness (VO2) | At baseline and 2 days after completing the 5-week intervention | VO2max was determined during the exercise testing. VO2max is the maximum of oxygen deliver and utilization. |
Countries
Taiwan
Outcome results
None listed