Inside a Neuron - Ex Vivo Evaluation of Cerebral and Cerebellar Neuronal Activity.

Inside a Neuron - Ex Vivo Evaluation of Cerebral and Cerebellar Neuronal Activity Through Electrophysiological and Imaging Techniques on Human Tissue from Surgical Activity.

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT06896331

Acronym

INSANE

Enrollment

Registered

2025-03-26

Start date

2024-09-17

Completion date

2027-09-30

Last updated

2025-03-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Human Neural Cells Functioning

Keywords

INSANE, neurons, human, glioma, brain, patch-clamp, HD-MEA

Brief summary

Understanding the functioning of human neurons has always been a key element, especially with regard to the translation of the inputs they receive into actions that determine human behavior. However, the vast majority of studies have been performed in animal models due to the difficulty in maintaining neuronal functions ex vivo. Recently, techniques have been illustrated that allow us to obtain slices of brain tissue removed during neurosurgical interventions. The patch-clamp and HD-MEA (high density - microelectrodes array) techniques, used until now for animal samples, can allow us to study the functioning of the neural cells that make up these slices. Furthermore, the exploration of the functioning of the aforementioned cells in pathological conditions can open the field for further therapeutic perspectives.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* patients with a brain/cerebellum primary or secondary tumor for whom at least partial resection has been planned.

Exclusion criteria

* patients with a brain/cerebellum primary or secondary tumor for whom a biopsy has been planned

Design outcomes

Primary

Measure	Time frame	Description
Primary endpoint	Through study completion, an average of 3 years.	Intrinsic and extrinsic properties of brain and cerebellum neurons in health condition. In particular, following element will be measured: * cell membrane capacity and resistance; * Sodium and Potassium currents; * cell firing frequency.

Secondary

Measure	Time frame	Description
Secondary endpoint	Through study completion, an average of 3 years.	Intrinsic and extrinsic properties of brain and cerebellum neurons in pathological condition. In particular, following element will be measured: * cell membrane capacity and resistance; * Sodium and Potassium currents; - cell firing frequency.

Countries

Italy

Contacts

Primary ContactFrancesco Guerrini, MD

f.guerrini@smatteo.pv.it+39 0382502780

Outcome results