Endothenial Dysfunction, Iron Status, Oxidative Stress
Conditions
Keywords
endotoxin, gut permeability, gastrointestinal function, iron status, oxidative stress
Brief summary
The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.
Detailed description
The principal objective of our study was to assess gut permeability after completing a 6000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 6000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1 hour of restitution. Parameters, such as I-FABP (intestinal fatty acid binding protein), LPS (lipopolysaccharide), LBP (lipopolysaccharide binding protein), claudin3, ferritin, transferrin, hepcidin, IL-6 (Interleukin-6), HO-1 (Heme Oxygenase 1), and ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) were determined using appropriate biochemical tests.
Interventions
OTHERexercise test
Participant perforemed 6000m ergometer test.
Sponsors
Poznan University of Physical Education
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 24 Years
Healthy volunteers
Yes
Inclusion criteria
* minimum five years of training, * five training sessions per week minimum, * total training time minimum of 240 minutes, * membership in the Polish Rowing Team, * finishing the 6000-meter ergometer test.
Exclusion criteria
* antibiotic therapy within the last three months, * probiotics and prebiotics within the last three months, * dietary regime, * gastrointestinal diseases.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. | ACSL4 concentration measured in blood \[pg/ml\] using commercially available enzyme-linked immunosorbent assays. |
| I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| LBP (lipopolysaccharide binding protein) to measure endotoxin | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays |
| Claudin 3 to measure tight junction leakage | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | claudin 3 concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| LPS (lipopolysaccharide) to measure endotoxin | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period | LPS concentration measured in blood \[EU/Ll\] using commercially available enzyme-linked immunosorbent assays |
| Lactic acid to measure fatigue after the race | Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test. | Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany) |
| Ferritin to measure iron status | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. | Ferritin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| Transferrin to measure iron status | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. | Transferrin concentration measured in blood \[mg/ml\] using commercially available enzyme-linked immunosorbent assays |
| Hepcidin to measure iron status | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. | Hepcidin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
| IL-6 (Interleukin-6) to measure inflammation | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. | IL-6 concentration measured in blood \[ng/l\] using commercially available enzyme-linked immunosorbent assays. |
| HO-1 (Heme Oxygenase 1) to measure oxidative stress | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. | HO-1 concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| protein | day before test, in the morning before the test | protein \[g \]of food intake measured by 24h dietary recall |
| carbohydrate | day before test, in the morning before the test | carbohydrate \[g \]of food intake measured by 24h dietary recall |
| fat | day before test, in the morning before the test | fat \[g \]of food intake measured by 24h dietary recall |
| energy | day before test, in the morning before the test | energy \[kcal \]of food intake measured by 24h dietary recall |
| fiber | day before test, in the morning before the test | fiber \[g \]of food intake measured by 24h dietary recall |
| body mass | in the morning before the test | Body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
| Body composition - water | in the morning before the test | % of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
| Body composition - fat | in the morning before the test | % of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
| Lean body mass | in the morning before the test | Lean body mass \[kg\] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). |
| Height | in the morning before the test | Prior to the exercise test, we measured the anthropometric parameters, including height (Seca 213 Hamburg, Deutschland) \[cm\] |
| Gastrointestinal sypthomps self reported scale | up to 1 hours after the test | 10 point scale were 10 is a outcome of the worst severity |
Countries
Poland
Outcome results
None listed