Immunocompromised Patients, Pneumocystis
Conditions
Keywords
immunocompromised patients, pneumocystis
Brief summary
Evaluate new diagnostic methods using qPCR on non-invasive samples, compared with reference techniques for the positive diagnosis of pneumocystis (PcP) in immunocompromised patients
Interventions
OTHERBiological sampling
Mouthwash, nasopharyngeal swab, blood sampling
Sponsors
Assistance Publique - Hôpitaux de Paris
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* adult patients with suspected pneumocystis * with an indication for bronchial fibroscopy for BAL or induced sputum * no opposition to the research from the adult patient or his or her next of kin research for patients unable to express their non-opposition
Exclusion criteria
* Pneumocystis treated for more than 72 hours * pregnancy/breast-feeding * adult patients deprived of liberty, protected
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Pearson correlation coefficient | At inclusion | Pearson correlation coefficient between the fungal load obtained by PCR on nasopharyngeal swabs nasopharyngeal swab and buccal lavage and that obtained by reference samples (induced sputum or BAL) in patients with confirmed PcP. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Fungal load by qPCR | At inclusion | Quantitative fungal load results obtained by qPCR on nasopharyngeal swab, mouthwash, induced sputum, BAL |
| Fungal load by serum PCR | At inclusion | Quantitative fungal load results obtained by serum PCR |
| Beta D glucan blood levels | At inclusion | — |
Countries
France
Contacts
CONTACTAgathe Vigouroux, MD
CONTACTJérôme Lambert, MD PhD
Outcome results
None listed