Alzheimer Disease
Conditions
Keywords
Alzheimer, mitochondrial function, metabolomic
Brief summary
This project aims to study on one hand the early mitochondrial alterations, common or specific, occurring in peripheral cells of patients with Alzheimer's disease or related dementia and, on the other hand, identify the metabolomic biomarkers that may be at the origin of mitochondrial disturbances associated with the disease. This will be the first study combining functional analyses of mitochondria and exploratory metabolomic assessments in the same cohort at early stages of the disease.
Detailed description
Study will be proposed to the patient of the Nice Memory center who had a lumbar puncture and who agreed on the storage of a sample of their cerebrospinal fluid. Two groups of patients will be compared: the Alzheimer"s disease patient with positive amyloid on the cerebrospinal fluid and the non Alzheimer patient with negative amyloid on the cerebrospinal fluid. there will also be a group of healthy volonteer with one blood sample Blood samples will be collected at inclusion and one year to obtain the mitochondrial metabolomic signature
Interventions
OTHERMMSE and blood draw
Each patient will be evaluated by Mini-Mental State Examination (MMSE) and will have a blood draw
Sponsors
Centre Hospitalier Universitaire de Nice
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Neurocognitive disorder with spontaneous complaint or reported for at least 6 months * Diagnostic process according to current Haute Autorité de Santé (HAS) recommendations. * cerebrospinal fluid biomarker dosage done within the study center and agreement to bio-collection of residual cerebrospinal fluid * Patient having agreed to sign the informed consent * Patient affiliated or beneficiary of a social security scheme For alzheimer group : diagnosis of Alzheimer's disease according to NIA-AA 2024 criteria (presence of a pathogenic amyloid process in the cerebrospinal fluid) For non alzheimer group : diagnosis of mild or major neurocognitive disorder according to Manuel diagnostique et statistique des troubles mentaux edition 5 (DSM-V) criteria, not linked to Alzheimer's disease (absence of pathogenic amyloid process in the cerebrospinal fluid) For healthy volonteers: * Adult patients * No history of neurological or psychiatric disorders * No complaints of memory problems or cognitive impairment * Mini-Mental State Examination (MMSE) score ≥ 27/30 * Clinical Dementia Rating - Sum of Boxes (CDR-SOB) equal to 0 * Patient affiliated with or covered by a social security scheme
Exclusion criteria
\- Vulnerable people
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mitochondrial oxygen consumption of peripheral blood cells | At Baseline | Comparison of the rate of mitochondrial oxygen consumption of peripheral blood cells between alzheimer (amyloid positive) and non-Alzheimer participant (amyloid negative) in pmol/min/cell mesured by Extracellular Flux Analyzer |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Mitochondrial metabolomic signature | At Baseline and at one year | Description of the panel of mitochondrial metabolites of peripheral blood cells according to the status of the participants (amyloid positive vs amyloid negative). |
| Mini Mental State Examination Scores for Cognitive Impairment | At Baseline and at one year | 30 questions, good response to a question = 1, bad response to a question = 0, global scoring between 0 and 30, Pathological threshold according to the socio-cultural level : without diploma : total score \<22 secondary education without the 3rd class : total score \<23 secondary education from 3rd class to the senior year class (without bachelor degree) higher education : total score \<26 |
Countries
France
Contacts
CONTACTJustine Lemaire
PRINCIPAL_INVESTIGATORAurélie MOUTON, MD,PhD
Centre Hospitalier Universitaire de Nice
Outcome results
None listed