Kidney Diseases
Conditions
Brief summary
This study will investigating the effects of glucagon on renal blood flow in humans using MRI technology. Glucagon, a hormone produced by the pancreas, plays a key role in regulating blood sugar levels. It has been shown to affect renal function, including electrolyte balance and blood flow, especially in conditions like type 2 diabetes where abnormal glucagon levels are common. The study aims to understand how glucagon affects regional blood flow in the kidneys, specifically the cortex and medulla, and whether these effects are mediated by glucagon receptors. The study will be conducted on 10 healthy male participants aged 20-60 years. It involves three test days where participants will receive either glucagon, glucagon with a GLP-1 receptor antagonist, or placebo. Blood flow, glomerular filtration rate, and other renal functions will be measured using MRI. The study seeks to clarify whether glucagon's effects on the kidneys are linked to changes in regional blood flow and to determine if these effects are mediated solely by glucagon receptors.
Interventions
DRUGGlucagon
Glucagon infusion at 5 ng·kg-¹·min-¹ from 0-30 minutes and 10 ng·kg-¹·min-¹ from 30-60 minutes.
DRUGGlucagon+Exendin9-39
Glucagon infusion at 5 ng·kg-¹·min-¹ from 0-30 minutes and 10 ng·kg-¹·min-¹ from 30-60 minutes, plus a GLP-1R antagonist, exendin 9-39 (900 pmol·kg-¹·min-¹), given intravenously from -30 to 60 minutes
DRUGSodium chloride
Placebo (0.9% NaCl).
Sponsors
Bispebjerg Hospital
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Caregiver, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
20 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Normal health confirmed through; Interview and Medical examination * Normal values with respect to blood concentrations of fasting plasma glucose, fasting plasma total cholesterol, fasting triglycerides, HDL, LDL, creatinine, liver function tests, and electrolytes * Informed consent
Exclusion criteria
* Immunosuppressive treatment in the previous 12 months * Alcohol abuse * Medical treatment with oral glucocorticoids, dipeptidyl peptidase-4 (DPP-4) inhibitors, or GLP-1 receptor agonists, which, in the opinion of the principal investigator, may interfere with glucose metabolism * Use of lithium * Medical treatment that affects insulin secretion or the renin-angiotensin-aldosterone system * Liver disease (ALT \> 2x normal value) * Renal impairment (serum creatinine \> 130 µM and/or albuminuria) * Individuals with severe claustrophobia * Individuals with MR-incompatible foreign bodies * Individuals with hypertension
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood perfusion of the kidney | 7 measurements of 10 minutes each from the start of infusion (time 0) and up to one hour (time 60). | we would like to measure blood perfusion of the kidneys over time |
Secondary
| Measure | Time frame |
|---|---|
| Blood flow of the renal arteries | 7 measurements of 10 minutes each from the start of infusion (time 0) and up to one hour (time 60). |
| blood oxygen saturation of the kidneys | 7 measurements of 10 minutes each from the start of infusion (time 0) and up to one hour (time 60). |
Outcome results
None listed