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OPTImisation and Standardization of CIRCulating Cell-free DNA Ex-traction Protocols in a Diagnostic Setting

Optimisation and Standardization of Circulating Cell-free DNA Ex-traction Protocols in a Diagnostic Setting

Status
Not yet recruiting
Phases
Unknown
Study type
Observational
Source
ClinicalTrials.gov
Registry ID
NCT06869070
Acronym
OPTICIRC
Enrollment
10
Registered
2025-03-11
Start date
2025-04-07
Completion date
2025-08-31
Last updated
2025-03-11

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy

Brief summary

comparison of blood collection tubes and cell-free DNA extraction to evaluate the best possible combination to obtain high quantity and quality cell-free DNA sample for NGS analyses

Interventions

None listed

Sponsors

University Hospital Tuebingen
Lead SponsorOTHER

Study design

Observational model
COHORT
Time perspective
PROSPECTIVE

Eligibility

Sex/Gender
ALL
Healthy volunteers
Yes

Inclusion criteria

* at least 18 years * no serious condition

Exclusion criteria

* younger than 18 years * serious health condition that leads to higher cfDNA content in blood

Design outcomes

Primary

MeasureTime frameDescription
cfDNA quantityBaseline, at enrollmentwe will assess the quantities in ng/µl of cell-free DNA extracted from plasma
cfDNA qualityBaseline, at enrollmentwe will assess the fragments length in nucleotides using TermoFisher's Tapestation from the extracted plasma cell-free DNA

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026