Exhaustion - Physiological
Conditions
Keywords
rowers, EPO, VEGF, hypoxanthine, glycogen, athletes
Brief summary
Intense physical exercise can lead to micro-damage to tissues and activation of inflammatory processes, the body's natural response to exercise-related metabolic stress. Monitoring inflammation and fatigue will enable the observation of athletes in terms of post-exercise regeneration and muscle adaptation to physical effort. The obtained results will allow for estimating the level of fatigue, minimizing the risk of injury, and will show the rate of post-exercise regeneration or its absence.
Detailed description
The study will involve 40 members of the Polish Youth Rowing Team, both sexes, aged 19-23. The study will be held on three dates related to the specifics of the athletes' training cycle, i.e. in the preparatory period (March), the competition period (June), and the detraining period (November). The participants will perform an intensive exercise test on a rowing ergometer (manufacturer: Concept II, USA), covering a distance of 2,000 meters in the shortest possible time. The study will involve 40 members of the Polish Youth Rowing Team, both sexes, aged 19-23. Each time, before starting the exercise test, a non-invasive anthropometric analysis will be performed using the TANITA MC-780MA body composition analyzer (tanita Japan), to determine the body composition: percentage of body fat, body fat mass, lean tissue mass, muscle mass, body water and body mass index (BMI). On the three indicated dates, before and after the stress test, capillary blood (ear lobe) from the cubital vein will be collected from the participants. Using immunoenzymatic ELISA tests, the following will be determined: EPO (erythropoietin), VEGF (vascular endothelial growth factor), CRP (C-reactive protein), glycogen, hypoxanthine (Hx). Glucose level will be determined using an Accu-Chek Active glucometer (Roche, Germany).
Interventions
OTHERPhysical effort
The examined rowers will be subjected to an effort on a rowing ergometer, consisting of covering a designated distance in the shortest possible time.
Sponsors
Poznan University of Physical Education
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
OTHER
Masking
NONE
Intervention model description
A group of rowers subjected to an efford on a rowing ergometer. The examined rowers group will be subjected to an effort on a rowing ergometer, consisting of covering a designated distance in the shortest possible time.
Eligibility
Sex/Gender
ALL
Age
19 Years to 23 Years
Healthy volunteers
Yes
Inclusion criteria
* members of the Polish Youth Rowing Team - competitors officially called up to the Polish national rowing team, * no musculoskeletal injuries that would prevent them from performing the exercise test, * written consent of the competitor to participate in the study.
Exclusion criteria
* anti-inflammatory drugs, other supplements in the last 3 months before starting the study, * failure to complete the test, * current injury, post-traumatic condition, illness, * lack of informed consent of the athlete.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| EPO (erythropoietin) level | Immediately before the ergometer test, immediately after and 3 hours after the test | Immunoenzymatic assay method using a diagnostic ELISA Kit |
| VEGF (vascular endothelial growth factor) level | Immediately before the ergometer test, immediately after and 3 hours after the test | Immunoenzymatic assay method using a diagnostic ELISA Kit |
| Hypoxanthine (Hx) level | Immediately before the ergometer test, immediately after and 3 hours after the test | Immunoenzymatic assay method using a diagnostic ELISA Kit |
| Glycogen level | Immediately before the ergometer test, immediately after and 3 hours after the test | Immunoenzymatic assay method using a diagnostic ELISA Kit |
| Glucose level | Immediately before the ergometer test, immediately after and 3 hours after the test | Using an Accu-Chek Active glucometer (Roche, Germany) |
Countries
Poland
Outcome results
None listed