Arterial Catheterization, Peripheral, Bacteremia, Biofilms, Tomography, Optical Coherence
Conditions
Brief summary
29.3% of bacteremias in intensive care units (ICU) are linked to vascular devices, including 7.7% to arterial catheters, with an influence on both morbility and mortality. It is now accepted that biofilm as a role on bacterial development on inner surface of vascular devices but there is yet a lack of clinical relevant data documenting a causal relation between biofilm formation and bacteremias. The investigators assume that a better structural and microbiological characterization of arterial catheter biofilm in ICU patients could help preventing bacteremias or have a more specific treatment when it appears.
Interventions
OTHEROCT-BIO-KTA cohort
Dynamic full-field optical coherence tomography (D-FF-OCT) analysis of arterial catheter sections before microbiological analysis of catheter biofilm
Sponsors
Centre Hospitalier William Morey - Chalon sur Saône
Centre Hospitalier Universitaire Dijon
Centre Hospitalier Macon
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patient or relative informed of the study and having declared their non-objection * Patient over 18 years old * Patient hospitalized in ICU with an arterial catheter
Exclusion criteria
* Patient unable to express consent * Patient whose collection of the arterial catheter is impossible or for whom storage at 4°C is impossible * Patient with arterial catheter removed outside the usual procedure of the ICU * Patient admitted in ICU with an arterial catheter already in place
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Biofilm structure type (ribbon-shaped or mushroom-shaped), measured with D-FF-OCT | Outcome measure is assessed 2 days following catheter removal | Provide a better understanding of the relation between structural and microbiological characterization of artherial catheter biofilm in ICU patients using D-FF-OCT |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Bacterial or fungal indentification | Outcome measure is assessed 2 days following catheter removal | Identification and enumeration of species - whether bacterial or fungal - by mass spectrometry from culture in agar medium of biofilm samples |
| Biofilm thickness, measured with D-FF-OCT | Outcome measure is assessed 2 days following catheter removal | Provide a better understanding of the relation between thickness and microbiological characterization of artherial catheter biofilm in ICU patients using D-FF-OCT |
| Biofilm dynamic signal distribution, measured with D-FF-OCT | Outcome measure is assessed 2 days following catheter removal | Provide a better understanding of the relation between dynamic signal distribution and microbiological characterization of artherial catheter biofilm in ICU patients using D-FF-OCT |
Countries
France
Contacts
CONTACTFlorian PERRAUD
CONTACTThomas MALDINEY
Outcome results
None listed