Muscle, Muscle Damage
Conditions
Keywords
DOMS, exercise, damaging, muscle, muscle recovery, vicia faba, fava bean, PeptiStrong
Brief summary
To investigate the effect of Vicia faba protein concentrate, a protein derived from Fava bean extract, on delayed onset muscle soreness (DOMS). Participants receive either 2.4g/day of PeptiStrong® supplement capsules or placebo capsules for 14 days until a strenuous exercise session. For the last 3 days, 2/3 of them stay on the same treatment and 1/3 of them switch from placebo to PeptiStrong®.
Detailed description
Delayed onset muscle soreness (DOMS) is the result of eccentric muscle contraction and is caused by small tears in the myofibrils. Damage to the muscle fibers causes pain, inflammation, and reduced range of motion which can delay recovery. Peak effects are often experienced 48 - 72 hours following the exercise session but can last up to 7 days post-exercise. Various modalities are utilized to reduce DOMS such as cryotherapy, massage, and compression. However, nutritional supplements have also been studied including Omega 3 fatty acids, creatine monohydrate, tart cherry juice, vitamin D, and probiotics. A recent study (Kerr, et al., 2023) investigated the effects of a protein supplement (PeptiStrong®) from Vicia faba protein concentrate, a protein derived from Fava bean extract. Results showed that 17 days of 2.4 grams of PeptiStrong® significantly improved muscle strength, reduced muscle fatigue, and reduced myostatin expression during the 72 hour recovery period compared to placebo following a strenuous bout of eccentric exercise. This study aims to repeat the methods of Kerr, et al. with the addition of females and a third experimental group. In addition to 17 days of supplementation with Vicia faba Hydrolysate or placebo, a third group will be given 14 days of placebo followed by 3 days of the Vicia faba Hydrolysate supplement to determine if short-term supplementation results in a similar benefit.
Interventions
OTHERPlacebo control capsules
2.4 grams (g) daily
OTHERPeptiStrong® supplement capsules with same appearance as placebo capsules
2.4 g daily
OTHERPeptiStrong® supplement capsules following muscle damaging exercise on Day 14
2.4 g placebo capsules for 14 days, then 3 days 2.4 g of PeptiStrong® capsules following one muscle damaging exercise session
Sponsors
PepsiCo Global R&D
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Males and females 18 - 45 years of age. * Willing to fast overnight on the 4 testing occasions. * Participants agree to abstain from taking additional supplements throughout the testing period, with particular emphasis placed upon protein-based products (e.g., whey or casein based protein powders; animal derived protein hydrolysates; natural supplements constituted of vegetative protein hydrolysates from rice, fava bean, pea, carrot, spirulina, broccoli, potatoes; marine derived secondary metabolite products or protein hydrolysates including Omega-3 capsules or DHA). * Moderately active (exercise 3-5 days per week) * Fluent in reading, writing, and speaking English * Participants agree to maintain their normal diet and perform only light to moderate exercise for the duration of the study. * BMI between 18.5 and 29.9 kg/m2 * Participants agree to refrain from consuming alcohol in the 48 hours leading up to a test day. * Willingness to complete questionnaires, records and diaries associated with the study and to complete all lab visits. * Refrain from any exercise from 48 hours prior to each test or blood draw. * Healthy as determined by General Health Questionnaire. * Non-smoker
Exclusion criteria
* Individuals that are glucose-6-phosphate-dehydrogenase deficient. The test product in this study contains fava bean extract which may produce favism in genetically susceptible individuals. * Alcohol or drug abuse in the past year.3. Testosterone or estrogen supplementation (not including women on oral contraceptives) * Pregnant or nursing or planning to become pregnant. * Participation in any other clinical trial in the past 30 days. Participation in any PepsiCo trial in the past 6 months * Volunteers with unstable medical conditions. * Any complaints that could interfere with the ability to exercise. * Individuals who are cognitively impaired or unable to give informed consent. * Any co-morbidities interacting with mobility or muscle metabolism of the lower limbs (e.g. arthritis, spasticity / rigidity, all neurological disorders, paralysis). * Creatine supplements, corticosteroids, NSAIDS, amino acids, injectible peptides, collagen, and nicotinamide (Vit B3/niacin). * Presence or history of neurological disorders or significant psychiatric illness. * Any condition the study investigator believes would interfere with eligibility following the study protocol, effect the study results, or put the subject at undue risk. * Participation in resistance or aerobic exercise within 48 hours of the test days. * Participation of \>3 high-intensity exercise sessions per week. * Undertake recovery methods such as sea swims, foam rolling, cryotherapy or excessive stretching during days 14-17. * Have been in contact with a suspected or confirmed case of COVID-19 in previous 14 days. * Are Hepatitis A- or B-positive or have had a sexual partner infected with hepatitis or HIV and not taking medication. * Are not employed by, or have a parent, guardian, or other immediate family member employed by a company that manufactures any products that compete with any Gatorade product. If you are unsure if a company would be considered a competitor to Gatorade, let the study investigator know the name of the other company and the nature of your relationship to that company before ou sign the informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Concentric knee extension and flexion strength | Following strenuous muscle damaging exercise on Day 14 | Assessed at an angular velocity of 60°/s through the range of 0-100° knee flexion using an isokinetic dynamometer (Biodex) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Exercise induced expression of markers of muscle stress | Days 14 at Baseline and 2 hrs post strenuous exercise, and on Days 16 and 17 at 10 minutes post exercise | Plasma concentrations of IL-6, IL-15, fractalkine, irisin, FGF21, myostatin, osteocrin / PEP- 2414 Page 9 of 32 1/29/25 V6 musclin, osteonectin/SPARC |
| Ratings of soreness, fatigue, pain, and tenderness. | Days 14, 15, 16, 17 based on the previous 24 hours | Rated on a 4-item Recovery survey, anchored from none to extreme |
| Fatigue index | Baseline Day 0, Day 16 and Day 17 for each strength test | Calculated from the strength tests using the following equation: (highest force - lowest force) / highest force |
Countries
United States
Contacts
Primary ContactKris Osterberg, PhD, RD
Backup ContactBrandon Shepherd, MS
Outcome results
None listed