Laryngeal Carcinoma
Conditions
Keywords
Laryngeal cancer, FosB(FBJ murine osteosarcoma viral oncogene homolog B) gene, Immunohistochemistry, Cox regression analysis
Brief summary
To determine whether there is statisstical difference in FosB (FBJ murine osteosarcoma viral oncogene homolog B) protein expression between laryngeal cancer tissue and adjacent tissue through immunohistochemistry methods. If there are differences, further the investigators will collect relevant patients information,such as clinical stages, pathological types, clinical prognosis and so on. ultimately analyze whether it is related to the expression of FOsB in laryngeal cancer tissue and adjacent tissues
Detailed description
First,the investigators will collect 100 to 200 cases of laryngeal cancer patients who have been finally diagnosed and treated in our institution in the past five years. Laryngeal cancer pathological tissues and adjacent tissues will be selected to make pathological sections. Use immunohistochemistry to confirm the differences in FosB protein between laryngeal cancer tissues and adjacent tissues, and clarify whether there are differences. secondly,if there are differences, further the investigators will collect relevant patients information,such as clinical stages, pathological types, clinical prognosis and so on. Review the clinical data of corresponding patients, including age, gender, smoking and alcohol history, clinical stages, pathological types and clinical prognosis, etc., and compare whether there are statistical differences. Finally, clinical prognostic data of relevant patients were collected through outpatient follow-up, online and telephone follow-up, to determine whether there is a correlation between Fosb expression in laryngeal cancer tissue and clinical prognosis.
Interventions
OTHERthe expression negative in laryngeal cancer tissue
Researcher will incorporate the positive expression of FOSB protein in laryngeal cancer tissues into the positive study group and vice versa into the negative study group by immunohistochemical methods!
OTHERthe expression positive in laryngeal cancer tissue
Researcher will incorporate the positive expression of FOSB protein in laryngeal cancer tissues into the positive study group and vice versa into the negative study group by immunohistochemical methods!
Sponsors
Yang ji
Study design
Observational model
OTHER
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Age
35 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
* laryngeal squamous cell carcinoma was diagnosed ,and biopsy was confirmed by two pathologists; * Primary tumor of Laryngeal ,and there was no distant metastasis; * Standard treatment such as surgery and Preliminarily-diagnosed parients; * full clinical and pathological data information and good follow-up; * there was no previous fatal diseases such as complicated tumors.
Exclusion criteria
* Secondary cancer or cancer with distant metastasis; * Patients who have not seen a doctor for the first time or have received surgery or radiotherapy or chemotherapy in other hospitals; * Clinical and pathological data were missing and loss to follow-up; * Patients who abandoned treatment or standard treatment procedures has not been completed; * The past medical history of other lethal diseases such as complicated tumors.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| the number of fosb gene positive expression cases in laryngeal carcinoma cells and adjacent tissues was determined. | one month | the number of fosb gene positive expression cases in laryngeal carcinoma cells and adjacent tissues was determined by immunohistochemical test of pathological sections. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| _Experimental result of western blot and PCR._Correlation analysis data between clinical information and experimental results.. | three months | Researcher will verify the effection of FosB gene on the proliferation and migration of Hep-2 laryngeal cancer cells through a series of basic experiments,such as Immunohistochemistry,western blot,PCR,and so on.clinical prognoostic information of patients was collected and the relationship with fosb gene expression was analyzed. |
Countries
China
Contacts
Primary ContactYang Ji
Outcome results
None listed