Investigating the Bactericidal Effect of Probiotics on Chromogenic Bacteria in Children An In Vivo and In Vitro Study

Effect of Probiotics on Chromogenic Bacteria and Extrinsic Stains in Children

Status

Completed

Phases

Phase 4

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT06834815

Enrollment

Registered

2025-02-19

Start date

2024-11-05

Completion date

2025-03-01

Last updated

2025-04-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Black Stains

Keywords

probiotics, chromogenic bacteria, black stains

Brief summary

To investigate the bactericidal effect of probiotics (Streptococcus salivarius M18 and Lactobacillus reuteri) on two types of bacteria (Aggregatibacter actinomycetemcomitans and Actinomyces naeslundii) causing black stains of teeth in vitro.

Detailed description

In vitro: An unstimulated salivary sample will be taken from patients having black stains. The two types of bacteria of each condition will be cultured, amplified, and measured in the laboratory from the saliva of these patients. After that, one type of probiotics will be applied to these bacteria separately and the same bacterial measurement will be done to evaluate the bactericidal effect of the probiotics on these bacteria, aiming to find the minimal inhibitory concentration of the probiotics.

Interventions

DRUGProbiotic with Prebiotic

After assigning the patients , all patients will submit an unstimulated salivary sample of 1ml will in sealed test tubes then stored in a deep freezer until microbiological evaluation, then they'll receive an oral prophylaxis session. * The patients will receive probiotics for 14 days given to them in a sealed box. * All patients will be followed up after 14 days and another salivary sample will be collected and sent for microbiological assessment.

Study design

Allocation

Intervention model

SINGLE_GROUP

Primary purpose

TREATMENT

Masking

NONE

Intervention model description

probiotics

Eligibility

Sex/Gender

ALL

Age

6 Years to 14 Years

Healthy volunteers

Yes

Inclusion criteria

* Patients with black stains on teeth scored via modified Lobene index scores 1-3. * Medically free patients

Exclusion criteria

* Stains other than black. * Patients on antibiotic treatment or any other medication for the last 2 weeks. * Patients allergic to prophylaxis powder. * Parents refusing to participate

Design outcomes

Primary

Measure	Time frame	Description
chromogenic bacterial count before and after probiotic application	two weeks	bacterial count

Countries

Egypt

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026