Root Canal Infection
Conditions
Keywords
chemical disinfection, chlorhexidine gluconate, gutta percha, sodium hypochlorite
Brief summary
This is a comparative experimental study to compare the efficacy of chemical disinfection agents , sodium hypochlorite and chlorhexidine gluconate in decontaminating gutta percha cones
Detailed description
The study design is a comparative study which was done to compare the efficacy of chemical disinfection agents on decontaminating the gutta percha cones . In this study, 124 standardized size-80 gutta-percha (GP) cones (Dentsply, Germany) were used to assess the efficacy of chemical disinfectants. The cones were first sterilized using ethylene oxide to ensure baseline sterility. Following sterilization, 124 cones were allocated into two experimental groups (n = 62 each) for contamination. group A . gp were immersed in 20ml of Enterococcus faecalis solution group B . gp were immersed in 20 ml of human saliva in patients undergoing root canal therapy samples were incubated at 37°C for 72 hours to ensure uniform and thorough contamination after incubation period cones were dried and divide into subgroups based on disinfectant agent applied . The cones were immersed in disinfectant agent for 1 min and 10 min . Cones were dried again and immersed in brain heard infusion broth samples were again incubated at 37°C for 72 hours The presence of bacterial growth was determined by assessing turbidity in the broth, indicating viable microorganisms.
Interventions
DRUGSodium Hypochlorite
Sodium hypochlorite used in 5% concentration for disinfection
DRUGChlorhexidine
Chlorhexidine 0.2% concentration for disinfection
Sponsors
Armed Forces Institute of Dentistry, Pakistan
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Caregiver, Investigator)
Eligibility
Sex/Gender
ALL
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy individual age between 20-40 patient needed root canal therapy
Exclusion criteria
* patient not willing to participate in study participants with systemic disease patients with neuromuscular disorder
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| effectiveness of disinfectants by assessing turbidity in broth using mann whitney test | 24 weeks | Following disinfection, all gutta-percha (GP) cones were dried again with sterile gauze and placed individually into sterile test tubes containing 20 mL of fresh brain heart infusion (BHI) broth. The test tubes were incubated at 37°C for 72 hours. The presence of bacterial growth was determined by assessing turbidity in the broth, indicating viable microorganisms. Quantitative analysis of turbidity data was performed using the Mann-Whitney test and Kruskal-Wallis test to evaluate the effectiveness of the disinfectants across the groups. |
Countries
Pakistan
Outcome results
None listed