PCOS (Polycystic Ovary Syndrome), Reproductive Age
Conditions
Keywords
menstrual disorders, chronic inflammation, insulin resistance, metabolic syndrome, Polycystic Ovary Syndrome
Brief summary
The study will involve measurements and comparisons of the concentrations of pro-inflammatory cytokines: IL-6, TNF-α, IL-18, as well as anti-inflammatory cytokine IL-4 in women with polycystic ovary syndrome (PCOS) and insulin resistance, women with polycystic ovary syndrome without carbohydrate metabolism disorders, and women without PCOS (control group).
Detailed description
Polycystic ovary syndrome (PCOS) is the most common endocrinopathy among women of reproductive age, which, in addition to menstrual disorders and infertility due to anovulation, is characterized by insulin resistance (IR). The metabolic disturbances associated with PCOS contribute to a higher incidence of cardiovascular diseases and endometrial cancer prior to menopause. The severity of IR in women with PCOS is linked to abdominal obesity. There is an urgent need to identify more precise and universal markers of insulin resistance than the HOMA-IR index or glucose tolerance tests, which are intended to identify existing disorders rather than predispositions. Additionally, there is a need to investigate the balance of pro-inflammatory and anti-inflammatory parameters in PCOS and their relationship with insulin resistance, which is responsible for the development of adverse health complications. The objective of this study is to measure and compare the concentrations of pro-inflammatory cytokines: IL-6, TNF-α, IL-18, and the anti-inflammatory cytokine IL-4 in women with polycystic ovary syndrome (PCOS) and insulin resistance, women with polycystic ovary syndrome without carbohydrate metabolism disorders, and women without PCOS (control group). Tests will be conducted using immunoenzymatic methods (ELISA) and the Erba XL biochemical analyzer. The concentrations of the substances under investigation will be measured and compared among the three study groups. Statistical analysis will be performed using SPSS Statistics software.
Interventions
DIAGNOSTIC_TESTMeasurement of interleukin-6 (il-6) concentration in blood serum
Measurement of fasting interleukin-6 (il-6) concentration in fasting venous blood serum
DIAGNOSTIC_TESTMeasurement of fasting tumor necrosis factor α (TNF-α) concentration
Measurement of fasting tumor necrosis factor α concentration in fasting venous blood serum
DIAGNOSTIC_TESTMeasurement of interleukin-18 (il-18) concentration
Measurement of interleukin-18 (il-18) concentration in fasting venous blood serum
DIAGNOSTIC_TESTMeasurement of interleukin-4 (il-4) concentration
Measurement of interleukin-4 (il-4) concentration in fasting venous blood plasma
Sponsors
Jagiellonian University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* women aged 18-45 years
Exclusion criteria
* previous removal of at least one ovary * treated diabetes of any type * diagnosed and treated metabolic diseases * diagnosed and treated autoimmune diseases * diagnosed and treated autoinflammatory diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Il-6 concentration | up to 6 months | Measurement and comparison of serum interleukin-6 (il-6) concentration expressed in \[pg/ml\] in three research groups |
| Il-18 concentration | up to 6 months | Measurement and comparison of serum interleukin-18 (il-18) concentration expressed in \[pg/ml\] in three research groups |
| Il-4 concentration | up to 6 months | Measurement and comparison of serum interleukin-4 (il-4) concentration expressed in \[pg/ml\] in three research groups |
| TNF-α concentration | up to 6 months | Measurement and comparison of serum tumor necrosis factor α (TNF-α) concentration expressed in \[pg/ml\] in three research groups |
Countries
Poland
Contacts
CONTACTIwona Gawron, Ph.D., M.D.
CONTACTRobert Jach, Prof., Ph.D., M.D.
STUDY_CHAIRKazimierz Pityński, Prof., Ph.D., M.D.
Jagiellonian University
Outcome results
None listed