PCOS, Reproductive Age
Conditions
Keywords
insulin resistance, polycystic ovary syndrome, metabolic syndrome, adipokines
Brief summary
The aim of the study is to compare the concentrations of leptin, resistin, and omentin-1 in women across three research groups: those with polycystic ovary syndrome without insulin resistance, those with polycystic ovary syndrome with insulin resistance, and women without polycystic ovary syndrome.
Detailed description
Polycystic ovary syndrome (PCOS) is the most prevalent endocrinopathy among reproductive-age women, characterized primarily by insulin resistance (IR), which has a prevalence of 35% to 80%. Metabolic disturbances associated with PCOS contribute to a higher incidence of cardiovascular diseases and endometrial cancer prior to menopause. The hyperinsulinemic-euglycemic clamp study is regarded as the gold standard for assessing insulin sensitivity; however, its clinical application is limited. Alternative assessments, such as the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) and fasting insulin or glucose tolerance tests, provide limited clinical utility. Therefore, there is a pressing need for the identification of more precise and universal markers of insulin resistance. There is a need to elucidate the precise role of adipokines in the etiology of PCOS and their association with insulin resistance, which is responsible for the development of adverse health-related complications. The aim of the study is to measure and compare the concentrations of selected adipokines: leptin, resistin, omentin-1 across three research groups: those with polycystic ovary syndrome without insulin resistance, those with polycystic ovary syndrome with insulin resistance, and women without polycystic ovary syndrome. The tests will be performed using immunoenzymatic methods (ELISA) and the Erba XL biochemical analyzer. The concentrations of the investigated substances will be measured and compared within the three research groups. Statistical analysis will be conducted using SPSS Statistics software.
Interventions
DIAGNOSTIC_TESTLeptin concentration in blood serum
Fasting plasma leptin concentration measurement with results expressed in pg/ml and comparison across all study arms
DIAGNOSTIC_TESTResistin concentration in blood serum
Fasting plasma resistin concentration measurement with results expressed in pg/ml and comparison across all study arms
DIAGNOSTIC_TESTOmentin-1 concentration in blood serum
Fasting plasma omentin-1 concentration measurement with results expressed in pg/ml and comparison across all study arms
Sponsors
Jagiellonian University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 18-45
Exclusion criteria
* previous removal of at least one ovary * treated diabetes of any type * diagnosed and treated metabolic diseases * diagnosed and treated autoimmune diseases * diagnosed and treated autoinflammatory diseases(Edited)Restore original
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Comparison of resistin concentration in three study groups | up to 6 months | Measurement and comparison of mean fasting plasma resistin concentration expressed in pg/ml in three study groups |
| Comparison of leptin concentration three study groups | up to 6 months | Measurement and comparison of mean fasting plasma leptin concentration expressed in pg/ml in three study groups |
| Comparison of omentin-1 concentration in three study groups | up to 6 months | Measurement and comparison of mean fasting plasma omentin-1 concentration expressed in pg/ml in three study groups |
Countries
Poland
Contacts
CONTACTIwona Gawron, Ph.D., M.D.
CONTACTRobert Jach, Prof., Ph.D., M.D.
STUDY_CHAIRKazimierz Pityński, Prof., Ph.D., M.D.
Jagiellonian University
Outcome results
None listed