Bloodstream Infections, Critically Ill Intensive Care Unit Patients, Sepsis
Conditions
Keywords
ddPCR, Sepsis, ICU
Brief summary
The purpose of this study is to investigate the efficacy of droplet digital PCR (ddPCR) in identifying pathogenic organisms in ICU sepsis patients, aiming to find a method that allows early identification of pathogenic organisms and dynamic surveillance in order to assess the association between pathogenic species and loads and clinical characteristics and outcomes.
Detailed description
This is a monocentric observational study. Patients admitted to the ICU with a first diagnosis of sepsis will be dynamically monitored for nucleic acid loads of pathogenic bacteria in the blood using ddPCR at different time points until the last blood sample is taken on day 28-30 or the patient is discharged from the ICU.
Interventions
None listed
Sponsors
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with first diagnosis of sepsis after ICU admission
Exclusion criteria
* Age under 18 years * Do not meet Sepsis 3.0 diagnostic criteria * Refusal to sign informed consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Nucleic acid load of pathogenic bacteria at different time points | ''Nucleic acid load of pathogenic bacteria in the blood versus time'' could have a time frame of ''0, 2-3, 4-5, 6-7, 8-9, 10-12, 13-15, 20-22, 28-30 days post admission to the ICU with the first diagnosis of sepsis''. |
Secondary
| Measure | Time frame |
|---|---|
| 28-day mortality | Survival status at 28 days after the first diagnosis of sepsis on admission to the ICU |
Countries
China
Contacts
Primary ContactYou Shang, prof.
Backup ContactXiaobo Yang, prof.
Outcome results
None listed