Breast Cancer, Colon Cancer, Endometrial Cancer, Esophageal Cancer, Gallbladder Cancer, Gastric Cancer, Kidney Cancer, Liver Cancer, Meningioma, Multiple Myeloma, Obesity, Ovarian, Ovarian Cancer, Pancreatic Cancer, Potmenopausal Breast Cancer, Prostate Cancer, Thyroid Cancer
Conditions
Keywords
obesity, cancer, bariatric surgery, proteomics, biomarkers, mass spectrometry
Brief summary
The expression of proteins in the blood of obese individuals is different from the expression of proteins in healthy individuals, and is also different in individuals after bariatric surgery. Therefore, this research aims to better understand protein expression in patients indicated for surgical treatment of obesity, evaluating the pre-operative and post-operative status. The objective of this research is to better understand protein expression in patients indicated for surgical treatment of obesity, evaluating the pre-operative and post-surgery status.
Detailed description
The objective of this study is to analyze the Proteomics of obese patients in the preoperative context of bariatric surgery to evaluate which are the up-regulated proteins - potentially related to oncogenic metabolic pathways - and also the down-regulated proteins in the context of obesity - potentially protective in the context of cancer. As well as, evaluate the variation of these proteins, as well as the metabolic pathways with which they are associated, after weight loss promoted by bariatric surgery, in the period of 01 year after this intervention. The proteomic study will be carried out based on the formation of pools organized based on the patients' pre- and postoperative similarities. Proteomic analyzes will be carried out on a high-performance liquid chromatography system (nanoUPLC) model Acquity Mclass, Waters. Coupled to a Quadrupole Mass Spectrometer-Time of Flight (QToF) Model Sunapt Xs, Waters. Finally, the raw data (raw mass spectra) will be analyzed by the PROGENESIS QI software (Waters) to identify the proteins.
Interventions
PROCEDUREbariatric surgery
sleeve gastrectomy
Sponsors
Universidade Federal de Pernambuco
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* patients over 18 years of age with a BMI above 35 kg/m² associated with comorbidity or BMI above 40 kg/m² without comorbidities,
Exclusion criteria
* Patients with a previous history of cancer; * Underage patients * Patients with other diseases predisposing to the development of cancer: cirrhosis, ulcerative colitis, Barrett's esophagus, etc. * Patients who have previously undergone bariatric surgery;
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of patients with reduced initially upregulated oncogenic proteins | 1 year after bariatric surgery | reduction of proteins potentially involved in oncogenic metabolic pathways, initially upregulated in the context of obesity |
Countries
Brazil
Contacts
Primary ContactLuiz Henrique A Bandeira, Medicine student
Backup ContactJúlia M Lins, medicine student
Outcome results
None listed