Obesity and Type 2 Diabetes
Conditions
Keywords
Stem Cell, Diabetes, Obesity, Regeneration
Brief summary
Femoral heads will be collected at University Hospital (London, ON, Canada) from individuals with type-2 diabetes, obesity, and no T2D/no obesity controls. The femoral head will be taken to the Robarts Research Institute (London, ON, Canada) for lineage-restricted progenitor cell expansion and analyses. There the femoral heads will be used to isolate three stem cell types - Multipotent Stromal Cells, Endothelial Colony Forming Cells, and Hematopoietic Progenitor Cells. The yield and cell surface markers expressed on these cells will be measured using flow cytometry. These cell types will then undergo further analyses of the secretome, tubule forming assays, vascular regenerative cell exhaustion assays, and will be transplanted into immune deficient mice with with femoral artery ligation. These experiments are to assess the differences in function and expression of these cell types between individuals with type-2 diabetes and/or obesity and/or healthy controls.
Detailed description
Femoral Heads will be extracted by Dr. Lanting (Department of Surgery - Division of Orthopaedics) at University Hospital, they will then be transferred to Robarts Research Institute where they will undergo cell isolation and analyses. Multipotent Stromal Cells, Endothelial Colony Forming Cells, and Hematopoietic Progenitor Cells will be extracted from the bone marrow of the femoral heads. These cells will be used to characterize the difference in regenerative progenitor cell number and potential dysfunction in individuals with type 2 diabetes and/or obesity compared to controls. Analysis will include growth kinetics, flow cytometry, Bio Luminescent Imaging and Laser Doppler Perfusion Imaging using a FAL mouse model.
Interventions
PROCEDUREHip Replacement
Total Hip Arthroplasty where the Femoral Head will be used to harvest stem cells post-extrication from the individual.
Sponsors
David Hess
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Must be 18+ years of age
Exclusion criteria
* Diagnosis of bone cancers * Diagnosis of Rheumatoid arthritis * Diagnosis of hematopoietic conditions * Diagnosis of avascular necrosis of the hip * does not fall within BMI requirements for experimental groups * Cannot read and/or speak in english
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cell Yield | Within 24 hours of surgery | Quantification of how many cells are harvested from each femoral head prior to expansion in vitro |
| Cellular Growth Kinetics | Time of cell harvest to 8 weeks post harvest | Quantification of the Growth Kinetics of each cell type during in vitro cell culture post cell harvest. |
| Cellular Phenotype | Time of cell harvest to 8 weeks post harvest | Analysis via flow cytometry of the phenotype of each cell type and their respective surface markers that are involved in regenerative pathways. |
Contacts
Primary ContactDavid A Hess, PhD
Backup ContactCaleb J Podgers
Outcome results
None listed