Liver Cirrhosis, Liver Failure, Liver Transplant Disorder
Conditions
Keywords
Oxidised albumin, Complement, Coagulation
Brief summary
The overall goal is to describe the oxidation state of albumin before, during and after liver transplantation. This is a pilot observational study in which the oxidative state in 10 patients undergoing liver transplantation will be described. Samples for analysing the oxidation state of albumin, general oxidative damage and anti-oxidant state, the activation of the complement system and factors involved in coagulation will be obtained before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30.
Interventions
None listed
Sponsors
Karolinska Institutet
Region Stockholm
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients on waiting list for liver transplantation
Exclusion criteria
* none
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Albumin quality | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. | Describe potential qualitative changes in albumin during the liver transplantation procedure. Non-oxidised, single oxidised and double oxidised albumin will be analysed by HPLC. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Effect of clinical factors on albumin quality after transplantation | On postop days 1, 2 and about 30. | Explore the impact of different clinical factors on albumin's oxidative state after transplantation. For this a multiple regression model will be used including clinical parameters; sex (male or female), age (years), MELD (Model for end-stage liver disease), bleeding during surgery (in liters), time of surgery (hours). |
| Graft outcome in relation to albumin quality | At days 1, 2 and about 30 days after transplantation | Explore of the oxidation state of albumin (percentage of albumin that is oxidised) is correlated with graft function (bilirubin levels in mg/L) |
| Oxidative stress in relation to albumin quality | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. | Explore is the oxidation state of albumin (percentage of albumin oxidised) is related to a general oxidative state measured by TBARS (Thiobarbituric acid reactive substances) measured as MDA (malondialdehydes) in nmol/ml. |
| Effect of clinical factors on albumin quality before transplantation | Before transplantation. | Explore the impact of different clinical factors on albumin's oxidative state before transplantation. For this a multiple regression model will be used including clinical parameters; sex (male or female), age (years), and MELD (Model for end-stage liver disease). |
| Binding capacity | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. | Explore whether the oxidation state of albumin (percentage of albumin oxidised) is affecting albumin's binding capacity measured by the ability of albumin in the sample to bind cobalt (mg/ml). |
| Complement system | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. | Explore how the complement system (C3bc in mg/ml) is affected during liver transplantation and how this is related to albumin quality (percentage of albumin oxidised) |
| Coagulation | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. | Explore how coagulation (Thrombin-antithrombin complex in microgram/ml) is affected during liver transplantation and how this is related to albumin quality (percentage albumin oxidised) |
| Anti-oxidant capacity in relation to albumin quality | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. | Explore is the oxidation state of albumin (percentage of albumin oxidised) is related to the total anti-oxidant capacity measured a the ability of the sample to reduce ferric ions (Fe3+) to ferrous ions (Fe2+) using a phenanthroline substance. |
Contacts
Primary ContactOlav Rooijackers, PhD
Backup ContactHåkon Haugaa, MD, PhD
Outcome results
None listed