Molar-Incisor Hypomineralization, Peridontal Disease
Conditions
Keywords
Gingival crevicular fluid, Matrix metalloproteinase 8, Matrix metalloproteinase 20
Brief summary
Background: To investigate the effect of molar incisor hypomineralization (MIH) on periodontal health, to evaluate the relationship between gingival crevicular fluid (GCF) volume, and GCF biomarkers such as matrix metalloproteinase-8 (MMP-8), matrix metalloproteinase-20 (MMP-20), and transforming growth factor-beta 1 (TGF-β1), and MIH. Methods: The study was conducted on 40 pediatric patients diagnosed with MIH (Study Group/SG), and 40 pediatric patients healthy for MIH (Control Group/CG). Clinical periodontal measurements (plaque index (PI), gingival index (GI), probing pocket depth (PPD), and GCF volume were performed. Concentrations of MMP-8, MMP-20, and TGF-β1 in GCF were determined using enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U test and T-test were used for comparisons between groups. Significance was set at p=0.05 for statistical analysis.
Interventions
OTHERClinical evaluation
In this study, it was aimed to measure the levels of MMP-8, MMP-20, and TGF-β1 in GCF together with the volume of GCF to investigate the effect of MIH on oral hygiene, and gingival health, and also to evaluate the relationship between MIH, and various biomarkers.
Sponsors
Recep Tayyip Erdogan University Training and Research Hospital
Study design
Observational model
CASE_CROSSOVER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
8 Years to 14 Years
Healthy volunteers
Yes
Inclusion criteria
* the ages of 8 and 14 who were diagnosed with MIH in their maxillary permanent first molars, * had healthy maxillary permanent first molars, * scored 3 (positive) or 4 (definitely positive) on the Frankl Behavior Scale, * had no mental or physical disability, * did not need urgent treatment and/or were not in pain, * agreed to participate in the study with their parents and signed the consent form.
Exclusion criteria
* Children with systemic, chronic, and/ or metabolic diseases, * under active orthodontic treatment, with developmental defects of enamel (excluding MIH), or born prematurely
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Periodontal indexes measurement | 1 day | PI, GI, and PPD measurements were performed to evaluate the periodontal health of the children participating in the study. |
| GCF measurement | 1 day | In the study, GCF was collected in the morning hours (between 09:00 and 11:00) one day after the patient's clinical periodontal measurements were complete. |
| Evaluation of MMP-8, MMP-20 and TGF-β1 Levels by ELISA Method | for three days from the beginning of the study | The levels of MMP-8 (Elabscience® EL-H1450, Biotechnology Co., Ltd, Wuhan, China), MMP-20 (Bioassay Technology Laboratory, Cat. No E4139Hu), and TGF-β1 (Elabscience® EL-H0110, Biotechnology Co., Ltd, Wuhan, China) in GCF samples were measured using ELISA kits. |
Countries
Turkey (Türkiye)
Outcome results
None listed