Biomarkers, Progression, Sarcoma
Conditions
Keywords
Sarcoma, OX40, Transcriptomics, Biomarker, CD134, prognosis
Brief summary
This study aims to investigate the expression of OX40 (CD134) in sarcomas and its impact on prognosis, with the goal of identifying novel biomarkers for early resistance detection and optimizing treatment strategies for sarcoma patients. Sarcomas are a highly heterogeneous group of malignant tumors, for which current treatment options are often suboptimal in certain patients, and effective biomarkers to guide therapy are lacking. As a T-cell costimulatory receptor, OX40 plays a significant role in immune regulation across various solid tumors and holds promise as a potential therapeutic target. The researcher's team has previously identified high OX40 expression in sarcomas through database analysis and validated the therapeutic efficacy of antibody-drug conjugates targeting OX40 in in vivo experiments using sarcoma cell lines in murine models. This study will evaluate the mRNA expression levels of OX40 in tumor tissues from sarcoma patients and assess OX40 protein expression using immunohistochemical staining. By integrating these findings with clinical and pathological data, the study will explore the potential of OX40 expression levels in tumor tissues as biomarkers for predicting treatment response and prognosis in primary bone tumors. The results aim to provide scientific evidence for the clinical application of OX40-targeted therapies and to propose novel therapeutic strategies to improve survival outcomes in sarcoma patients.
Interventions
DIAGNOSTIC_TESTIHC: Immunohistochemistry
Immunohistochemistry will be implied in tissue sections to test the expression of OX4O in sarcoma.
GENETICRNA-seq
RNA-seq will be implied in tissue sections to test the expression of CD134 RNA expression in sarcoma.
Sponsors
Peking University People's Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
8 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Aged 8 years or older. * Diagnosed with high-grade osteosarcoma or Ewing sarcoma, confirmed through clinical evaluation or histopathological examination. For patients with localized tumors or solitary pulmonary lesions, pathological confirmation is required; for patients with multiple pulmonary metastases, pathological examination is not necessary. * Treatment-naïve patients who have not received standard first-line chemotherapy regimens for osteosarcoma or Ewing sarcoma.(First-line chemotherapy drugs include high-dose methotrexate, anthracyclines, cisplatin, and ifosfamide. For Ewing sarcoma, the standard first-line regimen is VDC followed by IE.) * All other examination indicators suggest that the patient is eligible for standardized chemotherapy and surgical treatment for bone tumors.
Exclusion criteria
* Inability to undergo standardized chemotherapy and surgical treatment for bone tumors at this center. * Refusal to adhere to follow-up according to standardized diagnostic and treatment protocols. * Any condition that, in the investigator's opinion, may harm the participant or prevent them from meeting or complying with the study requirements.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| correlation of quantity of Protein OX40 Expression with clinical evaluation | 6 months | Clinical evaluation according to RECIST 1.1 |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| correlation of quantity of CD134 RNA with event-free survival | 6 months | correlation of quantity of CD134 RNA with event-free survival |
Countries
China
Outcome results
None listed