Age, TGF-β, Tissue Grafts, Vegf, Wound Healing
Conditions
Brief summary
This study aimed to assess the effects of age on wound healing on free gingival graft (FGG) surgery in the treatment of attached keratinized gingival deficiency by determining clinical and biochemical outcomes.
Detailed description
Aim of this study was to evaluate the effect of age on clinical and biochemical healing outcomes on FGG surgery in the treatment of attached keratinized gingival deficiency (≤2 mm) at mandibular anterior region. A total of 30 systemically healthy patients, divided into two groups and planned for FGG operations, were included in this study. These groups are; Group l : 15 patients in the age range of 20-35, group 2: 15 patients in the age range of 50-65. Gingival crevicular fluid and wound fluid samples were taken from these patients to analyze VEGF, EGF and TGF-B1 levels by ELISA. Wound fluid samples were taken baseline, 4,7, and 14 days after surgery. Clinical parameters included measurement of probing depth (PD), clinical attachment level (CAL), bleeding of probing (BOP), plaque index (PI), gingival index (GI) at 6 sites per tooth except the third molars were measured at baseline and l, 3 and 6 months after surgery. Keratinized gingiva width, keratinized gingiva thickness of the operation sites were measured baseline and 1,3 and 6 months after surgery. Vertical dimension of the graft, horizontal dimension of the graft, graft thickness were measured at baseline and 1,3,6 months after surgery. Graft area measurements were calculated on standard photographs by a Java-based analysis program (ImageJ, National Institutes of Health, Bethesda, MD) to determine the shrinkage rate of the grafts. The graft shrinkage ratio was calculated as the difference between baseline to 1-month, baseline to 3-months, baseline to 6-month graft site value. Data were analyzed using appropriate statistical tests.
Interventions
PROCEDUREFree gingival graft operation
Free gingival graft was harvested from palatal region and stabilized on the maxillary anterior recipient bed with sutures .
Sponsors
Marmara University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* having ≤2mm of keratinized gingiva in at least 1 mandibular anterior tooth * periodontally healthy with full-mouth bleeding and plaque score ≤ 10% * no use of any drugs that will affect periodontal tissue health and healing process * absence of any systemic condition or illness
Exclusion criteria
* smokers * previous periodontal surgery * a pathology in the apical region of the tooth * pregnancy, lactation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Shrinkage percentage (%) | from baseline to 1-,3-,6- months | Shrinkage percentage of the graft area due to the changes in graft dimensions |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| VEGF levels (pg/ml) | from baseline to 1-,3-,6- months | change in wound fluid VEGF levels from baseline to 1-,3-,6- months after surgery |
| EGF levels (pg/ml) | baseline to 1-,3-,6- months | change in wound fluid EGF levels from baseline to 1-,3-,6- months after surgery |
| TGFβ-1 levels (pg/ml) | from baseline to 1-,3-,6- months | change in wound fluid TGFβ-1 levels from baseline to 1-,3-,6- months after surgery |
Countries
Turkey (Türkiye)
Outcome results
None listed