Body Composition, Dermis Density, Dermis Thickness, Diet, Folic Acid Deficiency, Iron-deficiency, Micronutrient Deficiency, Nutrient Deficiency, Nutritional Status, Physical Activity, Skin Elasticity, Vitamin B 12 Deficiency, Vitamin D Deficiency
Conditions
Keywords
dietary intake, nutritional status, micronutrients
Brief summary
The aim of the study is to investigate the dietary habits of women of reproductive age (20-49 years). We aim to obtain epidemiological data on the intake and adequacy of key macronutrients and micronutrients, as well as to explore associations with various health indicators. Additionally, we will focus on the nutritional status of subgroups with specific dietary practices, such as vegetarians and vegans.
Detailed description
Key scientific challenges addressed in this study are to investigate the dietary habits and nutritional status of women of reproductive age, to provide epidemiological data on the nutrient intakes in this group (including intakes of key micronutrients) and investigate their relationship with various markers of health. We will evaluate dietary behaviours and special dietary practices among women of reproductive age in Slovenia, through age-representative sampling approach. We will also detailly assess dietary supplementation practices in this population group. Dietary intakes of macro and key micronutrients and nutritional/health status will be evaluated using a cross-sectional epidemiological study. Further we will investigate links between dietary behaviours with nutritional and health status in order to identify increased nutritional and health risk, as well as potential benefits of different dietary practices. We will particularly focus into following sub-groups of women: omnivorous women, that do not follow any special diet, women that follow vegetarian diet, and women that follow vegan diet. In order to investigate the status of the key micronutrients, standard blood biomarkers will be used. This will allow us to determine nutritional status for iron, folate, vitamin B12 and vitamin D. Body composition will be estimated using bioelectrical impedance spectroscopy (BIS). Other measurements of health-related parameters will include tissue protein density using ultrasonography. Study results will give insights into the nutritional status in general group of women of reproductive age and enable us to identify links between dietary behaviours with nutritional as well as health status. Through that approach we will be able to identify potential risk for deficiencies of nutrients in studied population group. Applying a highly multidisciplinary approach and building on previous research we will address the following: * evaluate dietary behaviours of women of reproductive age (20-49 years) through representative sampling approach. This will enable identification of diet related risks in this group. * determine dietary supplementation practices of women of reproductive age (20-49 years). This will allow us to better estimate intakes of specific nutrients, as supplements can be important dietary source of nutrients. * investigate nutritional status of women of reproductive age (20-49 years) in Slovenia with a combination of validated standard dietary assessment methods and blood biomarkers. This will enable also insights in nutritional status for key micronutrients, including iron, folate, vitamin B12 and vitamin D. * investigate links between dietary behaviours with nutritional/health status, providing information about sub-populations with higher health risks. To enable this, population of omnivorous subjects will be compared with those on vegetarian and vegan diet.
Interventions
Assessment of socio-demographic parameters, anthropometric and body composition parameters, food intake (dietary recalls, food propensity questionnaire) and nutritional status (blood biomarkers)
Dermis density and thickness will be measured using ultrasonography with Cortex DermaLab 20 MHz US probe.
Skin elasticity will be measured using Cortex DermaLab elasticity probe.
Body composition will be measured using Biostat Multiscan 5000.
Screening questionnaire: assessment of socio-demographic parameters, use of food supplements, health status, dietary habits
Sponsors
Study design
Eligibility
Inclusion criteria
* Age: 20 to 49 years * Gender: female * Agrees to participate in the study (has signed a consent form)
Exclusion criteria
* Menopause (absence of menstruation for at least 12 months, unless the absence is due to hormonal contraception) or post-menopause * Pregnancy Reasons for excluding a participant during the study: * Inability to participate in the study * Wishes to discontinue participation * Incomplete data provided Additional inclusion criteria for including subpopulations in the second part of the study: Vegetarians: * Have followed a vegetarian diet for at least 2 years and do not consume meat or fish more than once a month * Consume eggs and dairy products more than once a month Vegans: \- Have followed a vegan diet for at least 2 years and do not consume any animal products more than once a month
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Assessment of nutrient intake | 1 year | epidemiology of nutrients intake will be investigated from food records (dietary recalls, food propensity questionaires) \[g/day\] |
| Assessment of nutritional deficiencies | 1 year | epidemiology of nutritional deficiencies will be investigated using blood biomarkers |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Assessment of inadequate vitamin B12 status | 1 year | epidemiology of vitamin B12 status will be investigated using serum vitamin B12 concentration |
| Assessment of inadequate folate status | 1 year | epidemiology of vitamin folate status will be investigated using serum folate concentration |
| Assessment of inadequate iron status | 1 year | epidemiology of vitamin folate status will be investigated using serum ferritin concentration |
| Assessment of inadequate vitamin D status | 1 year | epidemiology of vitamin D status will be investigated using serum 25-OH-vitamin D concentration |
| Assessment of dermal thickness | 1 year | epidemiology of dermal thickness will be measured using 20 MHz ultrasonography in micrometers |
| Assessment of dermal density | 1 year | epidemiology of dermal density will be measured using 20 MHz ultrasonography as an intensity score 0-100 |
| Assessment of skin elasticity | 1 year | epidemiology of skin elasticity will be measured using Cortex DermaLab elasticity measurement |
| Assessment of body composition | 1 year | epidemiology of body composition will be investigated using Bioimpedance Spectroscopy (BIS) which measures at 50 frequencies ranging from 5 kHz to 1000 kHz giving values for impedance, resistance and reactance of the tissues (all in Ohms) from which body composition (proportion of lean and fat mass) values are calculated. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Body mass index (BMI) epidemiology of dietary intake of nutrients and fibre will be investigated from food records [g/day] | 1 year | Weight and height will be combined to report BMI in kg/m\^2 and providing insights into prevalence of overweight/obesity |
| Muscle strength | 1 year | Muscle strength will be measured as grip strength using dynamometer |
| Physical activity | 1 year | epidemiology of physical activity levels will be investigated using International Physical Activity Questionnaire (IPAQ) - Short Form |
Countries
Slovenia