Breastmilk Collection
Conditions
Keywords
Milk, Maternal, Virus, Transmission, Infectivity
Brief summary
Exclusive breastfeeding during the first six months of life and continued breastfeeding for at least 24 months constitute the optimal feeding method for infants and young children. However, breast milk and breastfeeding can be significant pathways for the transmission of certain viruses. The objective of this study is to examine the influence of human breast milk and its composition on viral infectivity and viral transmission of the HTLV-1 virus and arboviruses such as Zika virus, yellow fever, dengue, and tick-borne encephalitis.
Detailed description
Exclusive breastfeeding during the first six months of life and continued breastfeeding for at least 24 months constitute the optimal feeding method for infants and young children. However, breast milk and breastfeeding can be significant pathways for the transmission of at least three human viruses: HTLV-1, HIV, and human cytomegalovirus (CMV). To address the following main objective: to study the influence of human breast milk and its composition on viral infectivity and viral transmission of the HTLV-1 virus and arboviruses such as Zika virus, yellow fever, dengue, and tick-borne encephalitis.The study plans to collect milk samples from lactating women who are registered with the Lactarium of Île-de-France to donate their breast milk.
Interventions
OTHERCollection breast milk
Collection of a milk sample taken as part of women's donation
Sponsors
Institut Pasteur
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Age \> 18 years * Female gender * Lactarium Ile-de-France donors * Subjects covered by a Social Security scheme, excluding State Medical Aid No inclusion criteria: \- Women opposing participation in the study
Exclusion criteria
\- Positive HIV, HBV, HCV and HTLV serologies (Lactarium
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Study the influence of breast milk and its composition on viral infectivity and oral transmission of HTLV-1 and arboviruses such as Zika virus, yellow fever virus, dengue virus, and tick-borne encephalitis virus. | 36 months | Various viruses will be incubated with milk samples. The influence of milk on the different viruses will be studied. The capacity of these viruses incubated with milk to infect the target cells (immune or epithelial cells, depending on the virus) will be measured (measurement of the number of infected cells). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Determine the antiviral or proviral mechanisms of certain biological factors in breast milk. | 36 months | The level of proteins, carbohydrates and fatty acids will be measured in the milk using an infrared spectrometer. The aim is to find out whether the composition of the milk can influence its antiviral or proviral mechanisms. |
| Determine the role of breast milk in the mechanisms by which the relevant viruses cross an epithelium. | 36 months | Following the addition of viral particles or infected cells to the apical side of an in vitro intestinal model, the impact of breastmilk on crossing efficacy and underlying mechanisms will be assessed. Measurement of the number of viruses that cross the barrier of the intestinal epithelium in the presence of milk by comparing the number of viruses that cross the barrier of the intestinal epithelium without being incubated with milk. |
| Determine the influence of a few hours of incubation at 4°C (refrigeration) on the previously mentioned mechanisms. | 36 months | Milk samples will be incubated at 4°C. It has been demonstrated for several enveloped viruses, including the Zika virus, that refrigeration of breast milk (4°C) induces an antiviral effect. However, some experiments seem to indicate major differences in the kinetics of this effect, depending on milk factors. Kinetics will be measured. |
Countries
France
Contacts
Primary ContactAurore VIDY-ROCHE, PhD
Backup ContactPierre-Emmanuel CECCALDI, PhD
Outcome results
None listed