Hypoxia
Conditions
Brief summary
Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions.
Detailed description
Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions. It is hypothesized that the combined effect of these interventions will promote an additional performance and health improvement in this population at sea-level and altitude conditions, compared with the single effect.
Interventions
COMBINATION_PRODUCTNitrate + Hypoxia
The combined effect of nitrate supplementation and hypoxia training on both performance and health at sea-level and altitude conditions.
DIETARY_SUPPLEMENTPlacebo + Hypoxia
The combined effect of placebo supplementation and hypoxia training on both performance and health at sea-level and altitude conditions.
OTHERControl
The combined effect of placebo supplementation and normoxia training on both performance and health at sea-level and altitude conditions.
Sponsors
University of Trás-os-Montes and Alto Douro
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* trained male subjects, regularly involved in competitive running, cycling and/ or triathlon events (4-5 times per week) in the past five years
Exclusion criteria
* smoking or other chronic issues * using medication or dietary supplements in the last 3 months, or during the duration of the study * being exposed to altitude (≥2000 m) in the last 3 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Performance adaptations | Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention) | Time-to-task failure (time sustained) |
| Cardiovascular and neuromuscular adaptations | Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention) | Maximal oxygen uptake (VO2max: absolute and relative value) |
| Skeletal muscle adaptations | Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention) | Oxidative enzyme activity (cytochrome oxidase subunit IV), citrate synthase, transcription factors (HIF-1alpha and PGC-1alpha) and GLUT4 - assessed through standard enzymatic fluorometric assay and western bllotting |
| Haematological adaptations | Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention) | Blood pressure (Hmmg), mean arterial pressure (calculated as 1/3 \* systolic pressure + 2/3 \* diastolic pressure) |
| Oxidative stress and antioxidant markers adaptations | Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention) | Nitrotyrosine, malondialdehyde, ferric-reducing antioxidant power and superoxide dismutase concentrations - all assays on plasma sample analysed by spectrophometry |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Sleep and cardiac autonomic activity adaptations | Moment #1 (2 weeks pre-intervention - baseline) and moment #2 (4 weeks intervention) | Sleep duration (minutes) |
Countries
Portugal
Outcome results
None listed