Aging, Muscle Hypertrophy
Conditions
Keywords
aging, muscle mass, muscle strength, resistance exercise, sets
Brief summary
The study aimed to investigate the effects of resistance training volume manipulation on whole muscle hypertrophy among non-responders and responders to a low resistance training dose (single-set).
Detailed description
The magnitude of muscle hypertrophy in response to resistance training (RT) is highly variable between individuals (response heterogeneity). Manipulations in RT variables may modulate RT-related response heterogeneity; yet, this remains to be determined.
Interventions
The RT protocol was performed using a unilateral knee-extension machine. To reduce potential sources of bias to exercise order, subjects alternated the starting leg for each training session.
Sponsors
University of Sao Paulo
University of Sao Paulo General Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
SINGLE (Outcomes Assessor)
Intervention model description
Lower body unilateral design
Eligibility
Sex/Gender
ALL
Age
60 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* 60 years and older * not engaged in regular resistance and/or aerobic training for at least six months before the intervention
Exclusion criteria
* diagnosed type I diabetes mellitus * arrhythmia * noncontrolled hypertension * any major orthopedic musculoskeletal condition * use of any dietary supplement or drug with potential anabolic effects
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Quadriceps muscle cross-sectional area | 10 weeks | Quadriceps muscle CSA was obtained through magnetic resonance imaging. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Maximum dynamic strength | 10 weeks | Unilateral quadriceps 1-RM of both legs were assessed on a knee-extension machine |
Other
| Measure | Time frame | Description |
|---|---|---|
| Skeletal muscle RNA-sequencing | 10 weeks | RNA sequencing using an Illumina NovaSeq Platform with a standard paired-end 150 bp (PE150) dual indexing protocol |
| Dietary assessment | Before intervention | Dietary assemment using the USDA Automated Multiple-Pass Method |
| Mass spectrometry | 10 weeks | Mass spectrometry metabolomics |
| Muscle protein measures | 10 weeks | Upstream binding factor (UBF) muscle protein was quantified by Simple Western capillary immunoassay. |
Countries
Brazil
Outcome results
None listed