Healthy
Conditions
Keywords
Coffee, Caffeine, Energy expenditure, Caffeine metabolism
Brief summary
Obesity is a risk factor for chronic diseases, and improving energy expenditure is a way to prevent and treat obesity. The purpose of this project is to explore the relationship between the effect of coffee on energy expenditure and caffeine metabolism, which has important significance in the rational application of caffeine.
Detailed description
The investigators will study the relationship between the effect of coffee on energy expenditure and caffeine metabolism. The investigators will recruit healthy participants. Participants will be forbidden to consume stimulant drinks such as coffee for 24 hours before the experiment. When the participants arrive at the lab on the day of the experiment, they will sign an informed consent form, a health history questionnaire and a daily coffee consumption questionnaire. The changes of drinking water/coffee on total energy expenditure (TEE), body temperature, heart rate, blood pressure, electrocardiogram, memory，cognition, sleep time and so on will be observed. The concentration of caffeine and its metabolites in the blood and urine will be measured by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS).
Interventions
OTHERCaffeine content
Participants will drink the same volume of water or coffee, caffeine content is 2mg/kg by body weight.
Sponsors
Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
： * Healthy adults. * Must be able to drink coffee.
Exclusion criteria
* Diabetes. * Hypoglycemia. * Gout. * Osteoporosis. * Gastric ulcer. * Pancreatitis. * Intestinal obstruction. * Blood phobia. * Pathological hypo or hyper tension. * Impaired glucose tolerance. * Claustrophobia. * HIV,etc.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Total energy expenditure | 2 days | Total energy expenditure will be measured by Chamber (OMNICAL). |
| The concentration of caffeine and its metabolites in urine | 2 days | The contents of caffeine and it's metabolites will be measured by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS ). |
| The concentration of caffeine and its metabolites in serum | 1 day | The concentration of caffeine and its metabolites in serum will be measured by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS ). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Height | The morning of the first day of the experiment, 5 mins | Height will be measured by seca 217 stable stadiometer. Subjects wearing no shoes. |
| Body weight | The morning of the first day of the experiment, 5 mins | Fasting body weight will be measured by Bioimpedance Analysis (TANITA, MC-980). |
| Fat mass | The morning of the first day of the experiment, 10 mins | Fat mass will be measured by Magnetic Resonance Imaging (Shanghai united imaging, uMR 790 ). |
| Fat free mass | on the morning of the experiment, 5 mins | Fat free mass will be Bioimpedance Analysis (Tanita, MC-980). |
| Heart rate | 2 days | Heart rate will be measured by Polar heart rate belts. |
| Body temperature | 2 days | Body temperature of participants will be measured by body temperature patch (RIT-P02-MED). |
| Memory and cognitive tests | The morning of the experiment, 60 mins | Memory and cognitive tests will be tested by Cognitive Experiment Standard Toolbox (CEST). |
| Physical activity | 7 days | The physical activity of volunteers will be recorded by accelerometer (GT3XP-BTLE). |
| Sleep duration | 2 days | The sleep duration of volunteers will be recorded by polar watch. |
| Waist circumferences | The morning of the first day of the experiment,10 mins. | Waist circumferences will be measured using a whole body laser scanner (TG2000-F). |
| Hunger rating | 2 days | Hunger score will be measured on (Visual Analogue Scale) VAS by a pen and a paper. According to the scores of each participant, the highest score was 100 % hunger rate, and hunger rate (%) at other time points will be obtained from hunger score × 100 % / the highest score. |
| Electrocardiogram (ECG) | 2 days | ECG of participants will be measured by Amu ECG belt, the changes in P wave, PR interval, PR segment, QRS wave group, J point, ST segment, T wave, QT interval, and U wave will be observe. |
| Blood pressure | 2 days | Systolic and diastolic blood pressure of participants will be measured by Omron digital sphygmomanometer. |
Countries
China
Contacts
Primary ContactJohn R Speakman, PhD
Backup ContactHuanan Zhang, Master
Outcome results
None listed