Diffuse Large B Cell Lymphoma Refractory, Diffuse Large B Cell Lymphoma Relapsed, Follicular Lymphoma ( FL), High-grade B-cell Lymphoma (HGBCL), Primary Mediastinal Large B-Cell Lymphoma-Recurrent, Primary Mediastinal Large B-Cell Lymphoma-Refractory
Conditions
Keywords
CAR T
Brief summary
This is a Phase I, prospective, dose-finding study to evaluate the safety, persistence, and clinical activity of GF-CART01 in subjects aged 18-70 with relapsed or refractory (R/R) B-cell hematological malignancies and failure of two-line or more standard chemotherapies or auto-hematopoietic stem cell transplantation (HSCT).This study is a traditional 3+3 dose-escalation design to observe dose-limiting toxicity (DLT), establish the maximum tolerated dose(MTD)/recommended phase 2 doses (RP2D), and preliminary efficacy of GF-CART01. RP2D may equal to or lower than MTD
Interventions
BIOLOGICALGF-CART01
CAR positive viable T cells-Mid
Sponsors
GenomeFrontier Therapeutics TW Co., Ltd.
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
1. Subjects must be of age ≥ 18 years and ≤ 70 years 2. Subjects or their legal guardians must volunteer to participate in the study and sign the informed consent 3. Histologically confirmed diagnosis of Diffuse Large B-Cell Lymphoma - Not Otherwise Specified (DLBCL-NOS), follicular lymphoma (FL), Primary Mediastinal Large B-cell Lymphoma (PMBCL), or High-Grade B-Cell Lymphoma (HGBCL) per the world health organization (WHO) Classification Criteria for Lymphoma (2022) 4. Tumor cell surface expression of CD19 (+) and/or CD20 (+) by flow cytometry or immunohistochemistry staining 5. Relapsed, progressive or refractory disease (defined as have not achieved a complete response) after ≥ two lines of systemic therapy, including anti-CD20 antibody and anthracycline and/or Relapsed, progressive or refractory disease ( defined as have not achieved a complete response) after auto-HSCT 6. Subjects have any accessible PET-positive lesion or measurable CT-positive lesion per Lugano 2014 criteria 7. Adequate hematologic function: absolute neutrophil count (ANC) \> 1,000/μL, absolute lymphocyte count (ALC) \> 300/μL, platelet count ≥ 75,000/μL, hemoglobin ≥ 8.0 g/dL 8. Adequate hepatic function: alanine aminotransferase (ALT) ≤ 5 times upper limit of normal (ULN), aspartate transaminase (AST) ≤ 5 times ULN, total bilirubin ≤ 1.5 times ULN 9. Adequate renal function: blood estimated glomerular filtration rate (eGFR) ≥ 60 mL/min/1.73m2 (calculated by Modification of Diet in Renal Disease (MDRD) equation) 10. Adequate cardiac function: echocardiogram or multigated blood pool analysis (MUGA) shows left ventricular ejection fraction (LVEF) ≥ 50%; and no clinically significant electrocardiogram (ECG) findings 11. Adequate pulmonary function: no active infection in the lungs, blood oxygen saturation in indoor air ≥ 92% 12. No clinically significant pleural effusion determined by the investigators 13. Estimated survival time ≥ 3 months 14. Eastern Cooperative Oncology Group (ECOG) performance status 0 to 2 15. Willingness and ability to comply with protocol-stated requirements, instructions, and restrictions in the investigator's judgement
Exclusion criteria
1. Previously treated with any CAR T cell product or allogenic hematopoietic stem cell transplant (HSCT) 2. Known or suspected allergy, hypersensitivity, or intolerance to any ingredients of the investigational product (IP) 3. Known medical history or possible risk for taking contrast agent(s) for positron emission tomography (PET) and/or computed tomography (CT) scan 4. Received any other investigational product, cell therapy, or gene therapy within 12 weeks prior to the leukapheresis 5. Received any tyrosine kinase inhibitor within 2 weeks prior to the leukapheresis 6. Received any systemic steroid, immunotherapy (such as immune checkpoint inhibitors, T- cell transfer therapies, monoclonal antibodies), or chemotherapy within 4 weeks prior to the leukapheresis 7. Received any live vaccine from 2 weeks prior to the leukapheresis 8. Subjects with human immunodeficiency virus (HIV), syphilis, Hepatitis B or C infection: HIV-1 and HIV-2 antibody positive, syphilis antibody positive, both hepatitis B virus (HBV) DNA and hepatitis B core (HBc) antibody positive, or hepatitis C virus (HCV) antibody positive 9. Subjects with atrial or ventricular involvement by B-cell malignancies 10. Subjects with tumor mass requiring urgent treatment within 8 weeks prior to the leukapheresis, such as ileus, tumor lysis syndrome, or vascular compression 11. Subjects with severe disease or other uncontrolled diseases, such as coronary heart disease, angina pectoris, myocardial infarction, arrhythmia (urgent intervention indicated, life- threatening consequences, or hemodynamic compromise), cardiac angioplasty or stenting, unstable angina, cerebral thrombosis, cerebral hemorrhage, hypertension (systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg) 12. Unstable pulmonary embolism, deep venous embolism, or other major arterial/venous thromboembolism events that occurred within 6 weeks prior to the leukapheresis. If subjects receive anticoagulant therapy, the treatment dose and frequency must be stable for more than 14 weeks prior to the leukapheresis 13. History of craniocerebral trauma, disturbance of consciousness, epilepsy, cerebrovascular ischemia, cerebrovascular hemorrhagic diseases, dementia, cerebellar disease, or any autoimmune disease with central nervous system (CNS) involvement 14. Female subject of childbearing potential who: a. Has positive pregnancy test result; or b. Is lactating 15. Female subjects of childbearing potential, or male subject with female spouse/partner of childbearing potential, who refuses to adopt at least one form of birth control from the date of signing informed consent to 12 months after GF-CART01 infusion. Acceptable forms of birth control include: a. Established use of oral, injected or implanted hormonal methods of contraception b. Placement of an intrauterine device (IUD) or intrauterine system (IUS) c. Barrier methods of contraception: condom or occlusive cap (diaphragm or cervical/vault caps) 16. Any following situations that the investigators believe are not suitable for this trial and/or may increase the risk for subjects or interfere with the results of the study a. With severe active infections (except simple urinary tract infection and bacterial pharyngitis) b. With active central nervous system involvement by lymphoma, malignant cells in cerebrospinal fluid c. History of brain metastasis d. With uncontrolled malignancies e. Any toxicities due to prior therapy f. With any uncontrolled illness or a history of any illness
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Maximum tolerated dose (MTD) | From Day 1 to 28 days after cell infusion | Percentage of subjects with dose-limiting toxicity (DLT) from Visit 4 (Day 1) to Visit 9 (Day 29) after GF-CART01 cell infusion |
| Safety and tolerability of GF-CART01 | From Day 1 to 1 year after cell infusion | Percentage of subjects with treatment-emergent adverse events (TEAEs), ≥ grade 3 TEAEs, serious adverse events (SAEs), adverse events of special interest (AESIs) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Preliminary efficacy of GF-CART01-ORR | From Day 1 to 1 year after cell infusion | Measure overall response rate (ORR) from Visit 4 (Day 1) to Visit 16 (Year 1) by Lugano 2014 criteria |
| Preliminary efficacy of GF-CART01-DOR | From Day 1 to 1 year after cell infusion | Measure duration of response (DOR) from Visit 4 (Day 1) to Visit 16 (Year 1) by Lugano 2014 criteria |
| Pharmacokinetics of GF- CART01-Cmax | From Day 1 to 1 year after cell infusion | Measure the peak exposure (Cmax) of GF-CART01 after cell infusion |
| Pharmacokinetics of GF- CART01-Tmax | From Day 1 to 1 year after cell infusion | Measure the time to reach peak exposure (Tmax) of GF-CART01 after cell infusion |
| Pharmacokinetics of GF- CART01-pAUC | From Day 1 to 1 year after cell infusion | Measure the partial area under the curve (pAUC) of GF-CART01 after cell infusion |
| Pharmacokinetics of GF- CART01-Clast | From Day 1 to 1 year after cell infusion | Measure the last observed measurable concentration of GF-CART01 after cell infusion |
| Pharmacokinetics of GF- CART01-T1/2 | From Day 1 to 1 year after cell infusion | Measure the time required for concentration of GF-CART01 to decrease by 50% after cell infusion |
| GF-CART01 persistence | From Day 1 to 1 year after cell infusion | Measure the duration of GF-CART01 presence after cell infusion \- Time of Clast |
| Feasibility of GF-CART01 production | From leukapheresis to Day 1 | Measure the rate of successful GF-CART01 manufacture at Visit 4 (Day 1) \- Percentage of GF-CART01 products successfully meeting manufacturing criteria |
Countries
Taiwan
Contacts
CONTACTHoward Cheng
Outcome results
None listed