Satiety Hormones, Short-chain Fatty Acids, Satiety and Appetite, Intestinal Absorption, Metabolism, Glucose Homeostasis
Conditions
Brief summary
The goal of this crossover study is to evaluate the impact of short-chain fatty acids (SCFA) on the gut hormone release after administration in the small intestine or colon in healthy participants. The main question it aims to answer is whether the site of administration of SCFA affects the gut hormone release. On test days participants will ingest capsules filled with SCFA that are specifically delivered in the small intestine or the colon. Subsequently, blood samples are collected at regular time points.
Interventions
DIETARY_SUPPLEMENTSCFA
On test days participants will ingest the capsules during a standard no fiber breakfast. Blood samples will be collected at regular time points for 8 hours. A visual analogue scale that questions hunger and satiety will be completed at regular time points.
DIETARY_SUPPLEMENTmicrocristalline cellulose
On test days participants will ingest the capsules during a standard no fiber breakfast. Blood samples will be collected at regular time points for 8 hours. A visual analogue scale that questions hunger and satiety will be completed at regular time points.
Sponsors
KU Leuven
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* male and female * healthy * normal BMI (18.5-25 kg/m\^2) * age within 18-50 years
Exclusion criteria
* Chronic gastrointestinal disorders such as inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), celiac disease, chronic constipation (less than 3 stools a week) and chronic frequent diarrhoea (more than 3 stools a day) * Previous abdominal surgery, except from appendectomy * Being on a weight loss, gluten-free, lactose-free, or vegan diet * The donation of blood during the last 3 months or suffering from low blood haemoglobin levels * The use of antibiotics or other medication that affects the gastrointestinal tract 3 months preceding the study and/or during the study * The use of prebiotics or probiotics 2 weeks preceding the study and/or during the study * Pregnancy, lactation or wish to become pregnant during the study period * Previous or current substance/alcohol dependence or abuse (\> 2 units per day/14 units per week)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The release of gut hormones | 8 hours | Gut hormone GLP-1 (pmol/l) and PYY (pg/ml) concentrations will be quantified in the blood samples collected at different time points. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The glycemic and insulinemic response | 8 hours | The glucose (mg/dl) and c-peptide (nmol/l) concentration will be determined in blood samples collected at regular time points during the test day |
Countries
Belgium
Outcome results
None listed