Normal Weigth, Obesity
Conditions
Keywords
high-fat diet, high-calorie diet, leukocytes, LPS
Brief summary
After eating, blood composition changes, including increased triglycerides and glucose, which can trigger postprandial inflammation. Particularly with high-fat foods, pro-inflammatory lipopolysaccharide (LPS) increases. This activates leukocytes to release pro-inflammatory cytokines. In industrialized countries where snacking is common, many people spend the day in a postprandial state. Obese individuals tend to have chronic inflammation and show increased susceptibility to infections such as SARS-CoV-2. The main objective of the study is to investigate the response of leukocytes and the serum metabolome after food intake in individuals with obesity compared to healthy individuals, focusing on LPS as a key stimulus of innate immunity.
Interventions
OTHERTest meal
Test meal consisting of fries, chicken nuggets and eggs
Sponsors
University of Hohenheim
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
25 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Signed declaration of consent * from 25 years of age * BMI 20 kg/m2 - 25 kg/m2 or BMI 30 kg/m2 - 40 kg/m2 * Good venous conditions for blood collection * Sufficient understanding of the German language and sufficient mental state to understand information and instructions related to the study
Exclusion criteria
* Nicotine consumption * High-risk alcohol consumption (more than one standard glass per day for women, more than two standard glasses per day for men) * Antibiotic intake * Taking probiotics, prebiotics and synbiotics, unless taken for more than 90 days * Taking statins or other lipid-lowering medications (e.g., ezetimibe, fibrates) * Taking oral antidiabetics * Taking antacids * Manifest diabetes mellitus * Acute/unstable cardiovascular diseases * Acute inflammatory diseases * autoimmune diseases * kidney diseases * food allergy or food intolerance to food components of the test meal (e.g. eggs) * celiac disease * Pregnancy and lactation * Inability to consume the test meal orally * Placement in a clinic or similar facility due to official or court order (medical history) * Participation in another clinical study (current or within the last 30 days prior to study entry) * A medical condition or regular medication use that, at the investigator's discretion, does not permit study participation or evaluation of study parameters or consumption of the investigational product (individual decision)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| LPS | 30 min after test meal | LPS in Serum |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| HbA1c | immediately after the intervention | — |
| Serum cytokines and PAMPs | immediately after the intervention | — |
| Characterization of PBMCs | immediately after the intervention | The PBMCs are characterized by fluorescence-activated cell sorting (FACS) and RNA isolation. |
| Metabolome analyses | immediately after the intervention | — |
| Trimethylamine / SCFA in serum | immediately after the intervention | — |
| Fasting blood glucose | immediately after the intervention | — |
| lipoproteins | immediately after the intervention | — |
| Activability of PBMCs in vitro | immediately after the intervention | To analyze the reactivity of PBMCs, these are isolated, co-cultivated with bacteria and then the number of replicable bacteria is counted. |
Other
| Measure | Time frame |
|---|---|
| Fecal microbiome analysis | baseline |
| IgA in serum | immediately after the intervention |
| Microbiome RNA in serum | baseline |
Countries
Germany
Outcome results
None listed