Healthy
Conditions
Keywords
Immune, Cytokine, Flow Cytometry, Immunoendocrine
Brief summary
The objective of this project is to quantify the immunoendocrine response to acute stress events combining both physical and psychological challenges. This work will serve as a pilot project examining differential stress induction in response to exercise. Participants will have an instructor with prior military training lead a two-hour training session for 10 recreationally fit male volunteers. You will be randomly assigned to a high-intensity training group to participate in the military style training and a low-intensity training group who will participate in a low-intensity cardiovascular training protocol. Blood samples will be collected from you prior to and following the exercise session and these samples will be analyzed for endocrine measures as well as markers of immune function to include chemokines and cytokines.
Interventions
OTHERExercise
Subjects in the two groups preformed 90-100 minutes of exercise at different intensities.
Sponsors
University of Kansas
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
All subjects will be screened using a health-history questionnaire for contradictions to exercise by American College of Sports Medicine (ACSM) guidelines Inclusion Criteria: * Recreationally active * Between the ages of 18-40, * Healthy * Non-obese (BMI \<28 kg/m2) * Non-smoking * Free of metabolic or cardiovascular diseases.
Exclusion criteria
* Unhealed Fractures * Thrombophlebitis (blood clots) * Recent Surgery (within the last three months) * Recent Uncontrolled Bruising * Osteomyelitis (acute or chronic bone infection) * Myositis Ossificans (hardened scarring in muscle tissue of the thigh).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| T cell proliferation | 6.5 hours | Blood samples were collected using standard venipuncture technique. Blood samples were obtained at baseline, immediately post-exercise (0h), 1 hour post (1h), 4 hours (4h), and 6 hours (6h) post-exercise in heparin vacutainer tubes. Peripheral blood mononuclear cells (PBMC) were isolated using Ficoll-Paque PLUS density gradient centrifugation. Total T cells were purified from washed PBMC by E-rosetting. T cell proliferation was analyzed in response to co-stimulation through CD3+CD28 using plate-bound antibodies, or phytohaemagglutinin (PHA) treatment, or no simulation. Cells were incubated for 6 days at 37˚C in a humidified incubator with 5% CO2 and then analyzed by flow cytometry. Cells were analyzed by flow cytometry using anti-CD3-APC, and anti-CD152-PE antibodies immediately after CD3+ T cell isolation (0 h) and following 6 d in culture using an Accuri C6 flow cytometer. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Catecholamines (hormones) | 6.5 hours | Blood will be collected in a vial containing EDTA (6 ml) for analysis of catecholamines. Plasma for catecholamine (epinephrine and norepinephrine) analyses was obtained by centrifugation at 2000 g for 10 min at 4˚C. The supernatant was removed and stored at -80˚C until analysis. Catecholamine concentrations will be determined using BI-CAT ELISA kits and performed per the manufacturer's instructions. The samples were measured at 450 nm using a Synergy HT microplate reader. The blood samples will be obtained at the same time as for the primary outcomes (prior to and following exercise as well as one, four and six hours post-exercise. |
| Complete Blood Count | 6.5 hours | Peripheral blood will be collected in a vial containing EDTA (6 ml) for analysis of complete blood counts (CBC). These vials will be sent to a commercial clinical laboratory (Quest Diagnostics) for the CBC measures. The blood samples will be obtained at the same time as for the primary outcomes (prior to and following exercise as well as one, four and six hours post-exercise). |
| Cortisol | 6.5 hours | Blood will be collected in a vial containing EDTA (6 ml) for analysis of cortisol. These vials will be sent to a commercial clinical laboratory (Quest Diagnostics) for the cortisol measures. The blood samples will be obtained at the same time as for the primary outcomes (prior to and following exercise as well as one, four and six hours post-exercise). |
Countries
United States
Outcome results
None listed