Gingivitis, Periodontal Health, Periodontitis
Conditions
Keywords
gingival crevicular fluid, asprosin hormone, periodontal diseases, tumour necrosis factor alpha
Brief summary
The aim of this study was to investigate the levels of asprosin hormone and tumour necrosis factor alpha (TNF-α) in gingival crevicular fluids and to compare its level with those in saliva and serum in patients with gingivitis and periodontitis.
Detailed description
This study is investigating the level of asprosin hormone in gingival crevicular fluid of patients with periodontal diseases such as gingivitis and periodontitis, and observing the healthy patient in terms of the hormone levels, as well. 75 volunteer patients aged 18-45 years old were included in this study. These patients were divided equally to three groups: Group I; periodontal health, Group II; gingivitis, Group III; periodontitis.The body fluids including serum, saliva and gingival crevicular fluid were collected from this participants according to the proper protocol and their storage was provided under certain conditions. ELISA tests for asprosin and Tumour necrosis factor alpha were made in laboratory. Also, clinical measurements such as plaque index, bleeding on probing, probing pocket depth were recorded at first visit day of the participants. The data were analysed according to the different periodontal conditions, and correlated with asprosin hormone and tumour necrosis factor alpha level in gingival crevicular fluid, serum and saliva.
Interventions
None listed
Sponsors
Mustafa Kemal University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Exclusion criteria
* Those with any systemic disease * Individuals who take medication regularly * Pregnant or lactating women * Individuals with menopause and anthropause * Those who have undergone any periodontal treatment in the last six months * Smokers
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Asprosin level | Day 1 | Asprosin level (ng/mL) in gingival crevicular fluid, serum and saliva. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| TNF-alpha levels | Day 1 | TNF-alpha level (pg/mL) in gingival crevicular fluid, serum and saliva |
| Plaque index | Day 1 | The index was recorded from all participants as numerical between 0-3 |
| bleeding on probing | Day 1 | The bleeding on probing is stated by percentage. the ratio of the number of sites with bleeding gingiva on probing around teeth to total surfaces of all examined teeth |
| probing pocket depth | Day 1 | The parameters were recorded as mm in all participant. The probing pocket depth is measured from the marginal gingiva to bottom of pocket using a periodontal probe, from six surfaces of each tooth. |
| body mass index | Day 1 | the weight and height is combined to report BMI in kg/m2 |
Countries
Turkey (Türkiye)
Outcome results
None listed