Dental Pulp Disease, Neurogenic Inflammation
Conditions
Keywords
NPY, Dental Pulp, Dental Bleaching, Neurogenic Inflammation
Brief summary
Objective. This study assessed the neuropeptide Y (NPY) expression in healthy human dental pulp following tooth bleaching with three in-office hydrogen peroxide-based systems: Opalescence Boost© (Ultradent Products, South Jordan, UT), Pola Office© (SDI, Victoria, Australia), and Zoom© (Zoom! Bleaching System; Discuss Dental, Culver City, CA). Materials and Methods. Forty dental pulps were collected from healthy premolars scheduled for extraction for orthodontic reasons. Teeth were divided into four groups containing ten healthy premolars each: Control group (n= 10): the teeth were not exposed to dental bleaching agents (healthy pulps assessed for normal/basal NPY values). Pola Office system group (n= 10): application of Pola office (35% H2O2) for 8 minutes. Opalescent Boost system group (n= 10): application of Opalescent Boost (40% H2O2) for 20 minutes. Zoom system group (n= 10): application of Zoom! (25% H2O2 + cold blue light) for 15 minutes. The investigators rigorously followed the manufacturer's instructions for all bleaching systems. Following the extractions, the pulpal tissue was collected, placed in a 4% formaldehyde solution in Eppendorf tubes, and processed. NPY levels were measured using enzyme-linked immunosorbent assay (ELISA).
Interventions
OTHERH2O2 at 35% for 8 minutes
The investigators rigorously followed the manufacturer's instructions for all bleaching systems.
OTHERH2O2 at 40% for 20 minutes
The investigators rigorously followed the manufacturer's instructions for all bleaching systems.
OTHERH2O2 at 25% plus cold blue light for 15 minutes
The investigators rigorously followed the manufacturer's instructions for all bleaching systems.
Sponsors
Institucion Universitaria Colegios de Colombia
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
SINGLE (Investigator)
Masking description
The bleaching agent was applied using specific devices, with only the laboratory staff able to identify the corresponding group codes, which consisted of letters and numbers.
Intervention model description
Sample Collection: Teeth were anesthetized after tooth bleaching using 3% mepivacaine without a vasoconstrictor for maxillary premolars and an inferior alveolar nerve block for mandibular premolars. 10 minutes later, teeth were extracted within 5 minutes. For the control group, extractions also occurred ten minutes post-anesthesia. After extraction, teeth were rinsed with 5.25% sodium hypochlorite to eliminate any remaining periodontal ligament. Teeth were sectioned with a Zekrya bur, and pulp tissue was collected with a sterile excavator, placed in a 1 ml 4% paraformaldehyde Eppendorf tube, and stored at -70°C. ELISA: Fifty microliters of each standard, blank, and sample were added to wells in duplicate. A working solution with biotinylated antibody was added, and the plate was incubated at 37°C. After washing, horseradish peroxidase-conjugated solution was added, followed by substrate and stop solution. The optical density of each well was measured at 450 nm.
Eligibility
Sex/Gender
ALL
Age
18 Years to 27 Years
Healthy volunteers
Yes
Inclusion criteria
* Dental pulps were collected from non-smoking donors * Patients who need premolar extractions for orthodontic purposes. * These premolars must meet the following requirements: * Caries and restoration-free, * Complete root development (visually and radiographically confirmed), * Normal response to sensitivity testing.
Exclusion criteria
* Evidence of periodontal disease, * Traumatic occlusion * Previous orthodontic force application.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Neuropeptide Y expression | 8-20 minutes | Measurement of the increase or not of NPY expression compared to the control group (teeth without intervention) |
Countries
Colombia
Outcome results
None listed