Biomarkers / Blood, Physical Activity Level, Sleep, Type 2 Diabetes Mellitus (T2DM)
Conditions
Brief summary
In this study, we investigated the effects of physical activity level and sleep quality level on total antioxidant level (TAS), total oxidant level (TOS), Malondialdehyde (MDA) and Superoxide Dismutase (SOD) which are markers of oxidative stress, irisin, fibronectin type III-protein 5 (FNDC5).
Interventions
OTHERBiomarkers / blood
Analysis of routine biochemical parameters
OTHERQuestionnaire
Physical activity level will be assessed using the International Physical Activity Questionnaire Short Form (IPQI) and sleep quality will be assessed using the Pittsburgh Sleep Quality Index (PSQI). The data to be obtained from blood will be obtained by selecting blood samples that will be sent to biological waste.
Sponsors
Selcuk University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
30 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
* Being between 30-65 years old * Volunteering to participate in the study * Being literate * Being diagnosed with Type 2 Diabetes
Exclusion criteria
* Having a physical or mental illness that prevents communication * malignant disease, * infectious disease, * chronic obstructive pulmonary disease, * liver disease, * patients with kidney disease and joint or muscle disease will not be included
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| biomarkers derived from blood sera | 10 Minutes | blood glucose, total cholesterol, triglycerides, LDL and HDL analyzes |
| ELISA method of oxidative stress markers | 10 minutes | All reagents of the ELISA kits of the oxidative stress markers TAS, TOS, MDA and SOD will be brought to room temperature. Standards will be prepared according to the protocol and added to a 96-well plate with the samples. Buffer solution will be added to each well and incubated for 90 min at room temperature. After each step, necessary washes will be performed and finally chromogenic agents will be added and read spectrophotometrically. Although the timing and additions may vary according to the parameter, in general the procedure is as indicated. Each sample will be run 2 times for both TAS and TOS |
| Analysis of exercise-related markers by ELISA method | 10 minutes | — |
| International Physical Activity Questionnaire | 5 minutes | — |
| Pittsburgh Sleep Quality Index | 5 minutes | — |
Countries
Turkey (Türkiye)
Outcome results
None listed