Aging, Heart Failure
Conditions
Brief summary
The goal of this observational study is to profile changes in DNA methylation of circulating CD4+ T and CD8+ T cells from healthy young to aged with diagnosis of HFpEF, a particular phenotype of HF which is highly prevalent in aging. The main question it aims to answer is: -Do DNA methylation biomarkers help us to understand the role of inflammation in HFpEF during aging? Our goal is to provide a simple large-scale panel of epigenetic-sensitive biomarkers useful in aged patients with HF in the early natural history of the disease (HFpEF).
Interventions
OTHERRRBS
Measure of genomic regions with differentially methylated profiles and differentially expressed protein
Sponsors
University of Campania Luigi Vanvitelli
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Healthy subjects aged between 18 and 40 years * Aged subjects (≥65) without signs and symptoms of heart failure * Aged subjects (≥65) with diagnosis of heart failure with preserved ejection fraction (EF major than 50%)
Exclusion criteria
* Heart failure with reduced ejection fraction (EF minor than 40%) * Heart failure with mildly reduced EF (HFmrEF) (EF 41-49%) * History or diagnosis of cancer and inflammatory diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of differentially methylated positions and regions as assessed by RRBS | 6 months | We will identify a panel of positions and regions of the genome which are differentially methylated using as measure diff.meth more than or minor than 2 and p minor than 0.05 |
Countries
Italy
Contacts
Primary ContactGiuditta Benincasa, PhD
Outcome results
None listed