Intracanal Medication
Conditions
Keywords
Inflammation, Asiaticoside, Pulpal necrosis
Brief summary
The objective of this study is to investigate "clinically" the effectiveness of Asiaticoside based intracanal medication compared to the commonly used intracanal medicaments (calcium hydroxide Ca(OH)2 and Ledermix) on the levels of bacteria and inflammatory cytokines in root canals and periradicular tissues of teeth with apical periodontitis.
Detailed description
This study investigate "clinically" the effectiveness of Asiaticoside based intracanal medication compared to the commonly used intracanal medicaments (calcium hydroxide Ca(OH)2 and Ledermix) on the levels of bacteria and inflammatory cytokines in root canals and periradicular tissues of teeth with apical periodontitis.
Interventions
Calcium Hydroxide Intracanal medication
DRUGLeddermix Intracanal medication
Leddermix Intracanal medication
DRUGAsiaticoside Intracanal medication
Asiaticoside Intracanal medication
Sponsors
Mansoura University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
20 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
• Single-rooted with pulp necrosis and apical periodontitis.
Exclusion criteria
Receiving antibiotic treatment within the preceding 3 months. * Reporting systemic disease. * Teeth that could not be isolated with rubber dam. * Teeth with periodontal pockets deeper than 3 mm.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| counting the number of colonies forming units of bacterial cells Microbiology cell culturing (manual counting technique to the bacterial cells) [Time Frame: up to 7 months] | up to 7 months | counting the number of colonies forming units of bacterial cells Microbiology cell culturing (manual counting technique to the bacterial cells) \[Time Frame: up to 7 months\] |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| using enzyme-linked immunosorbent assay (ELISA test) to measure the amount of Inflammatory Cytokines (TNF-α), (IL-1β) and (PG2) ELISA test to measure the amount of tumor necrotizing factor (TNF-α), interleukin 1β(IL-1β) and prostaglandin E2 (PG2) | up to 25 weeks | using enzyme-linked immunosorbent assay (ELISA test) to measure the amount of Inflammatory Cytokines (TNF-α), (IL-1β) and (PG2) ELISA test to measure the amount of tumor necrotizing factor (TNF-α), interleukin 1β(IL-1β) and prostaglandin E2 (PG2) |
Countries
Egypt
Contacts
Primary Contactsalem alqahtani, master
Backup ContactGhada Abdelrazik
Outcome results
None listed