Healthy
Conditions
Keywords
Apigenin, Cryopreservation, Antioxidant, Human sperm, Oxidative stress
Brief summary
The goal of this clinical trial is to compare the effect of apigenin supplementation in freezing medium with no apigenin supplementation in freezing medium on post-thaw human sperm motility. It will also evaluate the sperm viability, total antioxidant capacity, and oxidative stress markers. The main questions it aims to answer are: * Does apigenin supplementation in freezing medium improve the post-thaw sperm motility? * Does apigenin supplementation in freezing medium improve the post-thaw sperm viability, total antioxidant capacity, and oxidative stress?
Detailed description
All normal semen samples from participants who give written informed consent and meet the eligibility requirements will be included in the study. Prepared semen samples were divided into 3 groups. 1. Pre-freezing sperm suspension 2. Frozen-thawed control without apigenin supplementation in freezing medium 3. Frozen-thawed sperm suspension with apigenin 0.2 mM supplementation in freezing medium Baseline sperm motility will be measured in pre-freezing sperm suspension. The last 2 groups will be stored in liquid nitrogen for at least one week. After thawing, the sperm suspensions will be evaluated for motility, viability, total antioxidant capacity, and oxidative stress markers.
Interventions
DIETARY_SUPPLEMENTApigenin
0.2 mM in freezing medium
Sponsors
Chulalongkorn University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
SUPPORTIVE_CARE
Masking
NONE
Intervention model description
1. Frozen-thawed control without apigenin supplementation in freezing medium 2. Frozen-thawed sperm suspension with apigenin supplementation in freezing medium
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men aged 18-45 years who can receive semen analysis services at the Reproductive Biology Unit, Chulalongkorn Hospital, Thai Red Cross Society * Normal semen analysis results according to the World Health Organization criteria (Volume ≥ 1.4 mL, Sperm concentration ≥ 16 million/mL, Total motility ≥ 42%, Progressive motility ≥ 30%) * Giving consent
Exclusion criteria
* A history of accidents, radiation to the testes, or a history of male reproductive system surgery * Undescended testis * Varicocele * A history of taking certain medications that affect sperm production, such as chemotherapy, hormones * Currently having sexually transmitted disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Percentage of sperm motility | At least 1 week of freezing | Sperm motility (total motility, progressive motility, non-progressive motility, immotile spermatozoa) will be reported in percentage. Compare results among freezing medium with apigenin group, control group, and baseline pre-freeze sperm suspension. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Percentage of sperm viability comparison between intervention and control group | At least 1 week of freezing | Viability test will be assessed by eosin-nigrosin staining technique |
Other
| Measure | Time frame | Description |
|---|---|---|
| Total antioxidant capacity comparison between intervention and control group | At least 1 week of freezing | Total antioxidant capacity will be assessed by ABTS assay |
| Oxidative stress markers comparison between intervention and control group | At least 1 week of freezing | Oxidative stress markers will be measured. e.g. reactive oxygen species |
Countries
Thailand
Outcome results
None listed