Autism Spectrum Disorder
Conditions
Keywords
Gut microbiota, Gluten free diet, Casein free diet
Brief summary
Assess the effect of a casein-free and gluten-free diet on gastrointestinal symptoms, clinical variables, and the composition of the intestinal microbiota in Mexican children with autism spectrum disorder.
Detailed description
The objective of this quasi-experimental pre-post study with time series is to evaluate the effect of a casein-free and gluten-free diet on gastrointestinal symptoms, clinical variables, and the composition of the intestinal microbiota in Mexican children with autism spectrum disorder. The main question it aims to answer is: Does a casein free and gluten free diet promote a reduction in gastrointestinal symptoms, an improvement in clinical variables, and a state of eubiosis in the composition of the intestinal microbiota in Mexican children with autism spectrum disorder? Participants will undergo: An intervention of a casein-free and gluten-free diet for 3 months. Sample collection before and after the nutritional intervention. Clinical evaluations.
Interventions
OTHERGluten free and casein free diet
Parents will be advised for three months to ensure that children with autism spectrum disorder follow a casein-free and gluten-free diet during this period.
Sponsors
University of Guadalajara
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
4 Years to 10 Years
Healthy volunteers
No
Inclusion criteria
Children between 4-10 years old diagnosed with autism spectrum disorder using the criteria established in the ADOS scale and the CARS.
Exclusion criteria
Children who have received: * Antibiotics * Antimycotics * Probiotic supplements * Prebiotic supplements * Any medication that may alter the microbial composition of the patient, minimum one month prior to sampling. * Children on a strict dietary regimen other than a casein-free and gluten-free diet. * Children with celiac disease or who present any gluten-related disorder. * Children with allergy to dairy products.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gut microbiota composition through Next Generation Sequencing (16SRNA) and bioinformatic analysis | 12 weeks | The composition of the intestinal microbiota will be sequenced, and gastrointestinal symptoms will be characterized before and after the nutritional intervention. |
Countries
Mexico
Contacts
Primary ContactNora M. Torres Carrillo, Ph.D.
Backup ContactPriscila I. Ibarra Montero, Ph.D.Student
Outcome results
None listed