Cancer, Cell-free DNA, DNA G-quadruplex, Methylation
Conditions
Brief summary
This study aims to evaluate the early diagnosis value of G-quadruplex DNA methylation in patients with cancer.
Detailed description
Global DNA hypomethylation is a hallmark of most cancers that occurs early during oncogenesis. We would like to investigate whether cancer-specific transcription factor binding to DNA G-quadruplex drives DNA hypomethylation in cancer. In this observational study, we would like to clarify whether DNA hypomethylation around G-quadruplex loci in cell-free DNA could be detected in early-stage cancer patients from healthy donors with high sensitivity and specificity.
Interventions
OTHERG4 DNAm test
Detection of DNA methylation (DNAm) around G-quadruplex loci in cell-free DNA from blood.
Sponsors
Zhongnan Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
42 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Healthy volunteers identified as asymptomatic during medical examination; * Age≥42 years old; * Voluntarily participate in this study and sign the informed consent.
Exclusion criteria
* History of malignancy or having achieved complete remission following tumor surgery; * Certain autoimmune diseases (e.g., systemic lupus erythematosus, Sjögren's syndrome, antiphospholipid syndrome, rheumatoid arthritis, Crohn's disease, IgA nephropathy, myasthenia gravis, dermatomyositis, immune thrombocytopenic purpura, etc.); * Pregnancy; * Acute trauma (including surgical procedures), sudden onset organ failure (e.g., resulting from a car accident, fulminant hepatic failure, acute pancreatitis, severe pneumonia due to influenza virus), or diagnosis of sepsis; * Previous receipt of allogeneic blood transfusion, transplant surgery, or allogeneic cell therapy; * Volunteers who cannot provide informed consent.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| G-quadruplex DNAm score | 2024.04.30-2024.09.30 | Detection of DNAm around G-quadruplex loci in cell-free DNA from blood. |
Countries
China
Outcome results
None listed