Physiological Stress
Conditions
Brief summary
The purpose of our study is to investigate the effects of supplemental mannose on sera of humans who are healthy. We will perform proteomics studies to elucidate changes to glycan structure and protein level changes. We hypothesize that we may find that mannose supplies more properly glycosylated protein substrate and helps expedite protein synthesis, that excess mannose leads to alternative glycan structures which confer improved stability, or that there are new sites that become glycosylated after mannose supplementation.
Detailed description
Subjects will be enrolled and a baseline blood sample taken. Subjects will take mannose supplements for 1 week (3-4 packets of a standard dose in water), then a follow up blood sample will be taken. Subject blood will be analyzed by proteomic analysis for 1) change in glycan structure or 2) other protein based approaches to measure changes in levels of proteins.
Interventions
DIETARY_SUPPLEMENTMannose
Mannose is a sugar monomer that is important in the glycosylation of many proteins. The concentration of mannose in mammalian plasma is \ 50-100 μM, which is primarily derived from N-glycan processing. Taking dietary mannose supplements can raise the levels of plasma mannose by 3- to 5-fold. There are currently no known adverse effects of mannose supplementation in humans.
Sponsors
University of Texas Southwestern Medical Center
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* At least 18 years of age * No known medical conditions * Taking no medications or nutritional supplements * Agree to the study and provide informed consent
Exclusion criteria
* Less than 18 years of age * Known medical diagnosis, including pregnancy * Presently taking medications and/or nutritional supplements * Do not provide consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes to glycan structure | ~1 month | Glycopeptide mass spectrometry will be applied to the major glycoproteins present in serum. |
| Change in glycoprotein levels | ~1 month | Standard laboratory methods such as ELISA in either research or clinical laboratory settings (Roche Cobas or similar platform). |
Countries
United States
Outcome results
None listed