Rhabdomyolysis
Conditions
Brief summary
This study focuses on detecting the possible beneficial effect of Lactoferrin (Lf) administration on the kidney and the heart of glycerol-induced rhabdomyolysis (RM) in rats and its relation to ferroptosis and AMPK/Nrf2/ HO-1 signalling pathway. The main questions it aims to answer are: 1. Is Lf administration 30 min. before or after RM induction in rats beneficial to kidneys and heart? 2. When administered to glycerol-induced rats, does Lf act by upregulating the AMPK/Nrf2/ HO-1 signalling pathway and inhibiting ferroptosis?
Interventions
DRUGLactoferrin
an iron-binding protein
DIAGNOSTIC_TESTiron
Iron in kidney and heart tissues
DIAGNOSTIC_TESTMDA, TAC, and GSH
Oxidative stress levels in kidney and heart tissues
DIAGNOSTIC_TESTNrf2
Nrf2 expression in kidney and heart tissues
DIAGNOSTIC_TESTAMPK
AMPK levels in heart and kidney tissues
DIAGNOSTIC_TESTHO-1
HO-1 levels in kidney and heart tissues
DRUGglycerin
GLY intramuscular injection for rhabdomyolysis induction
Sponsors
Aswan University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Caregiver, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
10 Weeks to 12 Weeks
Healthy volunteers
No
Inclusion criteria
* • Twenty-four healthy male Wistar Albino rats weighing from 250 to 300 g
Exclusion criteria
* No
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Nrf2 expression | 2 months | Nrf2 expression in heart and kidney tissues |
| AMPK levels | 2 months | AMPK levels in kidney and heart tissue homogenate |
| HO-1 levels | 2 months | HO-1 levels in kidney and heart tissue homogenate |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| iron levels | 2 months | Iron levels in heart and kidney tissue homogenate |
| GSH levels | 2 months | GSH levels in heart and kidney tissue homogenate |
| MDA levels | 2 months | MDA levels in heart and kidney tissue homogenate |
| TAC levels | 2 months | TAC levels in heart and kidney tissue homogenate |
Countries
Egypt
Outcome results
None listed