Exercise Induced CD8+ T Cell Recruitment and Characterization of Function and Phenotype of These Cells
Conditions
Brief summary
Healthy human subjects perform supramaximal exercise with venous blood samples obtained before and up to 60 minutes after the exercise bout.
Detailed description
Healthy, human subjects perform 3 x 30 seconds of supramaximal exercise. Venous blood samples were obtained before and up to 60 minutes after the exercise bout. The blood was used for absolute count of lymphocytes and monocytes per volume blood, and for isolation of CD8+ T cells. It was also used for blood gas analysis. The CD8+ T cells were characterized using flow cytometry, and then cultured for 7 days with CD8+ activation beads. The cells were counted and analyzed for viability at day4 and day8. RNAseq was performed on CD8+ cells isolated before and directly after exercise. CD8+ T cells from Day 1 pre and post exercise and then after 7 days of culture, were analyzed for IFNgamma release using Elispot, and for cytotoxicity against HEK cancer cells in vitro. Flow cytometry of CD8+ T cells was performed on fresh CD8 T cells pre and post exercise, and then on day4 and day8 after culture. SeaHorse metabolic assay was done on Day1 CD8 T cells obtained pre and post exercise.
Interventions
PROCEDUREExercise
3 x 30 seconds of supramaximal exercise
Sponsors
Karolinska Institutet
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 36 Years
Healthy volunteers
Yes
Inclusion criteria
* Be used to physical exercise and be able to perform strenous exercise Be able to attend the experimental procedure for the 90 minute duration of the study
Exclusion criteria
* Smoking Pregnant Have any medical condition requiring medical treatment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| CD8 T cell absolute nr after exercise | Before, directly after, 60 minutes after exercise | CD8 nr |
| CD8 T cell cytotoxicity against cancer cells | Cryopreserved CD8 cells from Day 1 and Day 8 of the experiment, obtained before and after exercise | CD8 T cell lysis of cancer cells |
| CD8 T cell pheontype after exercise | Before, directly after, 30 minutes and 60 minutes after exercise | Flow cytometry analysis of CD8 T cells |
Countries
Sweden
Outcome results
None listed