Male Infertility
Conditions
Keywords
ICSI, Male infertility, PGT-A, TESE
Brief summary
There is evidence indicating that sperm plays a role in determining blastocyst ploidy status. In our study, we aim to compare the outcomes of preimplantation genetic testing for aneuploidy (PGT-A) and the subsequent clinical outcomes following transfers of euploid embryos. This comparison will be conducted between cases where ejaculated sperm and cases where testicular sperm were used for intracytoplasmic sperm injection (ICSI), specifically focusing on severe male factor infertility patients.
Interventions
PROCEDURETesticular sperm extraction (TESE)
Surgical procedure used to retrieve sperm directly from the testicles of men who have low sperm production or no sperm in their ejaculate
OTHEREjaculate
Ejaculate processing for ICSI
Sponsors
Ganin Fertility Center
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Cases planned to have ICSI PGT-A cycles * Males diagnosed as severe male factor infertility * Normo responder female partners
Exclusion criteria
* Leukocytospermia * Presence of varicocele. * Known genetic abnormality * Use of sperm or oocyte donors * Use of gestational carrier * Presence of any of the endometrial factors that affect embryo implantation such as hydrosalpings, adenomyosis or previous uterine infection * Any contradictions to undergoing in vitro fertilization or gonadotropin stimulation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Clinical pregnancy rate | 6-9 weeks post-embryo transfer | defined as the presence of a fetal heartbeat or gestational sac 6-9 weeks post-embryo transfer |
| Euploidy rate | 15 days post ICSI | Defined as the proportion of euploid blastocysts |
| Ongoing pregnancy rate | 12 weeks of gestation | defined as a positive heartbeat on ultrasound beyond 12 weeks of gestation |
| Miscarriage rate | after 6 weeks of gestation | defined as pregnancy loss following confirmation of clinical pregnancy |
| Aneuploidy rate | 15 days post ICSI | Defined as the proportion of aneuploid blastocysts |
| Low mosaic rate | Time Frame: 15 days post ICSI | Defined as the proportion of low mosaic blastocysts |
| High mosaic rate | Time Frame: 15 days post ICSI | Defined as the proportion of high mosaic blastocysts |
| Implantation rate | 6-9 weeks post-embryo transfer | defined as the percentage of successfully implanted embryos |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cleavage rate | 3 days | Defined as the proportion of cleaved embryos on day 3 |
| Blastocyst development rate | 5-6 days | Defined as the proportion of blastocysts formed on day 5 or 6 |
| Fertilization rate | 1 day | Defined as the proportion of fertilizaed oocytes |
Countries
Egypt
Outcome results
None listed